In vitro evaluation of the effect of a novel immunosuppressive agent, FTY720, on the function of feline neutrophils

Yi-Je Chen, Andrew E. Kyles, Clare R. Gregory

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective - To use in vitro assays to evaluate the effects of a novel immunosuppressive agent, FTY720, on biological functions (migration, phagocytosis, and production of reactive-oxygen species [ROS]) of feline peripheral neutrophils and determine the cytotoxic effects of FTY720 on feline peripheral neutrophils. Sample population - Peripheral neutrophils obtained from 8 healthy cats. Procedure - Peripheral neutrophils were isolated from blood sampl es obtained from the 8 cats and exposed to the phosphorylated form of FTY720 (FTY720-P). A fluorescence-based in vitro evaluation of migration was performed. Phagocytosis of microbes and production of ROS were evaluated by use of a 2-color flow cytometry system. Samples of whole blood obtained from the cats were incubated with various concentrations of FTY720-P fluorescein-labeled Staphylococcus aureus, and dihydroethidium. Cytotoxic effects were evaluated by use of propidium iodide staining. Results - Addition of FTY720-P caused a slight non-sig nificant decrease in phagocytosis and production of ROS by feline peripheral neutrophils. Migration activity of feline peripheral neutrophils was significantly increased by the addition of FTY720-P. Addition of FTY720-P at concentrations considered for clinical use did not increase the death rate of feline peripheral neutrophils. Conclusions and clinical relevance - FTY720 does not inhibit critical functions of feline peripheral neutrophils in vitro.

Original languageEnglish (US)
Pages (from-to)588-592
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume67
Issue number4
StatePublished - Apr 2006

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immunosuppressive agents
Felidae
Immunosuppressive Agents
neutrophils
Neutrophils
cats
Phagocytosis
phagocytosis
Reactive Oxygen Species
Cats
reactive oxygen species
cytotoxicity
Fingolimod Hydrochloride
In Vitro Techniques
Propidium
Fluorescein
blood
fluorescein
Staphylococcus aureus
Flow Cytometry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro evaluation of the effect of a novel immunosuppressive agent, FTY720, on the function of feline neutrophils. / Chen, Yi-Je; Kyles, Andrew E.; Gregory, Clare R.

In: American Journal of Veterinary Research, Vol. 67, No. 4, 04.2006, p. 588-592.

Research output: Contribution to journalArticle

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