In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Elizabeth A. Wahl, Fernando A Fierro, Thomas R. Peavy, Ursula Hopfner, Julian F. Dye, Hans Günther Machens, José T. Egaña, Thilo L. Schenck

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.

Original languageEnglish (US)
Article number108571
JournalBioMed Research International
Volume2015
DOIs
StatePublished - 2015

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Stem cells
Mesenchymal Stromal Cells
Scaffolds
Wound Healing
Biocompatible Materials
Wounds and Injuries
Chorioallantoic Membrane
Regenerative Medicine
Chitosan
Dermis
Fibrin
Regeneration
Swine
Collagen
Clinical Trials
Cytokines
Skin
Survival
Tissue regeneration
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Wahl, E. A., Fierro, F. A., Peavy, T. R., Hopfner, U., Dye, J. F., Machens, H. G., ... Schenck, T. L. (2015). In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. BioMed Research International, 2015, [108571]. https://doi.org/10.1155/2015/108571

In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. / Wahl, Elizabeth A.; Fierro, Fernando A; Peavy, Thomas R.; Hopfner, Ursula; Dye, Julian F.; Machens, Hans Günther; Egaña, José T.; Schenck, Thilo L.

In: BioMed Research International, Vol. 2015, 108571, 2015.

Research output: Contribution to journalArticle

Wahl, EA, Fierro, FA, Peavy, TR, Hopfner, U, Dye, JF, Machens, HG, Egaña, JT & Schenck, TL 2015, 'In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds', BioMed Research International, vol. 2015, 108571. https://doi.org/10.1155/2015/108571
Wahl, Elizabeth A. ; Fierro, Fernando A ; Peavy, Thomas R. ; Hopfner, Ursula ; Dye, Julian F. ; Machens, Hans Günther ; Egaña, José T. ; Schenck, Thilo L. / In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. In: BioMed Research International. 2015 ; Vol. 2015.
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