In vitro evaluation of endothelial cell loss using the neusidl corneal inserter

David Davis-Boozer, Mark A. Terry, Mark A. Greiner, Jeff Holiman, Hisham A. Saad, Asem A. Alqudah, Jennifer Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

PURPOSE:: To determine the immediate endothelial cell loss (ECL) resulting from insertion of a precut donor button using the Neusidl Corneal Inserter (NCI) and compare it with the previously published ECL resulting from insertion of a folded donor button using non-coapting forceps. METHODS:: Ten corneas were precut for Descemet stripping automated endothelial keratoplasty and trephinated to a diameter of 8.0 mm (n = 5) or 8.5 mm (n = 5). Each tissue was placed onto the platform of a new NCI spatula and inserted into a cadaveric whole globe through a 5.2 mm incision. The tissue was carefully removed and stained with trypan blue and alizarin red to detect damaged endothelium. ECL was estimated using Adobe Photoshop planimetry. Mean ECL was compared with previously reported studies of forceps insertion with a one-sample t test, using SPSS v. 19. Geographic patterns of ECL were also documented. RESULTS:: Mean ECL was 15.6% (95% confidence interval, 13.8-17.4). We were unable to detect a difference in ECL compared with previous insertion methods studied (P < 0.001). The pattern of damage from the NCI was different than that previously seen with forceps insertion. CONCLUSION:: Immediate endothelial damage resulting from use of the NCI for insertion of Descemet stripping automated endothelial keratoplasty tissue is comparable with that seen with a standard forceps technique, but with a different damage pattern.

Original languageEnglish (US)
Pages (from-to)479-482
Number of pages4
JournalCornea
Volume32
Issue number4
DOIs
StatePublished - Apr 2013

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Endothelial Cells
Surgical Instruments
Descemet Stripping Endothelial Keratoplasty
Endothelium
Trypan Blue
In Vitro Techniques
Cornea
Confidence Intervals

Keywords

  • Adobe Photoshop
  • corneal transplantation
  • DSAEK
  • endothelial cell loss
  • Neusidl Corneal Inserter

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Davis-Boozer, D., Terry, M. A., Greiner, M. A., Holiman, J., Saad, H. A., Alqudah, A. A., & Li, J. (2013). In vitro evaluation of endothelial cell loss using the neusidl corneal inserter. Cornea, 32(4), 479-482. https://doi.org/10.1097/ICO.0b013e318274a72b

In vitro evaluation of endothelial cell loss using the neusidl corneal inserter. / Davis-Boozer, David; Terry, Mark A.; Greiner, Mark A.; Holiman, Jeff; Saad, Hisham A.; Alqudah, Asem A.; Li, Jennifer.

In: Cornea, Vol. 32, No. 4, 04.2013, p. 479-482.

Research output: Contribution to journalArticle

Davis-Boozer, D, Terry, MA, Greiner, MA, Holiman, J, Saad, HA, Alqudah, AA & Li, J 2013, 'In vitro evaluation of endothelial cell loss using the neusidl corneal inserter', Cornea, vol. 32, no. 4, pp. 479-482. https://doi.org/10.1097/ICO.0b013e318274a72b
Davis-Boozer D, Terry MA, Greiner MA, Holiman J, Saad HA, Alqudah AA et al. In vitro evaluation of endothelial cell loss using the neusidl corneal inserter. Cornea. 2013 Apr;32(4):479-482. https://doi.org/10.1097/ICO.0b013e318274a72b
Davis-Boozer, David ; Terry, Mark A. ; Greiner, Mark A. ; Holiman, Jeff ; Saad, Hisham A. ; Alqudah, Asem A. ; Li, Jennifer. / In vitro evaluation of endothelial cell loss using the neusidl corneal inserter. In: Cornea. 2013 ; Vol. 32, No. 4. pp. 479-482.
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