In vitro evaluation of a customized solution for use in attenuating effects of ischemia and reperfusion in the equine small intestine

L. M. Van Hoogmoed, J. R. Snyder, Jorge Nieto, F. A. Harmon

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective-To determine whether a customized solution could attenuate the effects of low-flow ischemia and reperfusion injury of the equine jejunum. Sample Population-A segment of jejunum obtained from 21 healthy adult horses. Procedure-A segment of jejunum was maintained in an isolated extracorporeal circuit, and arterial flow was reduced to 20% of baseline for 40 minutes (ischemia) followed by 60 minutes of reperfusion. In 1 group, a customized solution was infused at a rate of 1 ml/min during low-flow ischemia and 3 ml/min during reperfusion. In a second group, the solution was infused at the same rate during low-flow ischemia, but it was infused at a rate of 7 ml/min during reperfusion. Control groups received lactated Ringer's solution administered at the same rates as for the customized solution. Various metabolic, hemodynamic, histologic, and permeability variables were recorded. Results-A lower flow rate during reperfusion (3 ml/min) had a beneficial effect, compared with lactated Ringer's solution or the higher flow rate (7 ml/min). Use of the solution at this rate resulted in less histomorphologic injury and reduced mucosal permeability to albumin. Conclusions and Clinical Relevance-Use of a customized solution at a lower flow rate during reperfusion appeared to have a protective effect on equine jejunum when administered IV during low-flow ischemia and reperfusion.

Original languageEnglish (US)
Pages (from-to)1679-1686
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume62
Issue number11
StatePublished - 2001

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ischemia
Horses
Small Intestine
Reperfusion
small intestine
Ischemia
Jejunum
horses
jejunum
Permeability
permeability
Reperfusion Injury
In Vitro Techniques
Albumins
hemodynamics
Hemodynamics
protective effect
albumins
Control Groups
Wounds and Injuries

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro evaluation of a customized solution for use in attenuating effects of ischemia and reperfusion in the equine small intestine. / Van Hoogmoed, L. M.; Snyder, J. R.; Nieto, Jorge; Harmon, F. A.

In: American Journal of Veterinary Research, Vol. 62, No. 11, 2001, p. 1679-1686.

Research output: Contribution to journalArticle

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