In vitro effects of erythromycin, lidocaine, and metoclopramide on smooth muscle from the pyloric antrum, proximal portion of the duodenum, and middle portion of the jejunum of horses

Jorge Nieto, Peter C. Rakestraw, Jack R. Snyder, Nick J. Vatistas

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Abstract

Objective - To evaluate effects of erythromycin, lidocaine, and metoclopramide on smooth muscle of the pyloric antrum (PA), proximal portion of the duodenum (PD), and middle portion of the jejunum (MJ) of horses. Sample Population - Strips of smooth muscle from 7 horses. Procedure - Isolated muscle strips were suspended in a bath and attached to isometric force transducers. Once stable spontaneous contractions were observed, agents were added. Isometric stress responses were compared with the amplitude of spontaneous contractions. Results - A single dose of erythromycin to the PA increased contractile amplitude (CA) for the longitudinal smooth muscle (mean ± SEM, 76 ± 16 g/cm2) but decreased CA for circular smooth muscle (-79 ± 23 g/cm2). The inhibitory effect was decreased by tetrodotoxin, N(G)-nitro- L-arginine methyl ester, and a vasoactive intestinal peptide antagonist. Erythromycin increased CA for the MJ, which was maximal at 10-4M (171 ± 36 g/cm2). Lidocaine increased CA for the PD, which was maximal at 10-4M (60 ± 5 g/cm2). Metoclopramide increased the CA, which was maximal at 10-4M for the PA (75 ± 26 g/cm2), PD (279 ± 33 g/cm2), and MJ (456 ± 59 g/cm2). Conclusions - Regional differences in responses to erythromycin, lidocaine, and metoclopramide were evident in the gastrointestinal tract of horses. Metoclopramide increased CA in all tissues used, whereas erythromycin inhibited CA in circular smooth muscle but stimulated CA in longitudinal smooth muscle from the PA. Inhibition is caused by stimulation of inhibitory nerves and is mediated, in part, by nitric oxide and vasoactive intestinal peptide.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume61
Issue number4
StatePublished - Apr 2000

Fingerprint

metoclopramide
Pyloric Antrum
lidocaine
Metoclopramide
erythromycin
Erythromycin
Jejunum
Lidocaine
jejunum
duodenum
Duodenum
smooth muscle
Horses
Smooth Muscle
horses
vasoactive intestinal peptide
Vasoactive Intestinal Peptide
tetrodotoxin
NG-Nitroarginine Methyl Ester
Tetrodotoxin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "In vitro effects of erythromycin, lidocaine, and metoclopramide on smooth muscle from the pyloric antrum, proximal portion of the duodenum, and middle portion of the jejunum of horses",
abstract = "Objective - To evaluate effects of erythromycin, lidocaine, and metoclopramide on smooth muscle of the pyloric antrum (PA), proximal portion of the duodenum (PD), and middle portion of the jejunum (MJ) of horses. Sample Population - Strips of smooth muscle from 7 horses. Procedure - Isolated muscle strips were suspended in a bath and attached to isometric force transducers. Once stable spontaneous contractions were observed, agents were added. Isometric stress responses were compared with the amplitude of spontaneous contractions. Results - A single dose of erythromycin to the PA increased contractile amplitude (CA) for the longitudinal smooth muscle (mean ± SEM, 76 ± 16 g/cm2) but decreased CA for circular smooth muscle (-79 ± 23 g/cm2). The inhibitory effect was decreased by tetrodotoxin, N(G)-nitro- L-arginine methyl ester, and a vasoactive intestinal peptide antagonist. Erythromycin increased CA for the MJ, which was maximal at 10-4M (171 ± 36 g/cm2). Lidocaine increased CA for the PD, which was maximal at 10-4M (60 ± 5 g/cm2). Metoclopramide increased the CA, which was maximal at 10-4M for the PA (75 ± 26 g/cm2), PD (279 ± 33 g/cm2), and MJ (456 ± 59 g/cm2). Conclusions - Regional differences in responses to erythromycin, lidocaine, and metoclopramide were evident in the gastrointestinal tract of horses. Metoclopramide increased CA in all tissues used, whereas erythromycin inhibited CA in circular smooth muscle but stimulated CA in longitudinal smooth muscle from the PA. Inhibition is caused by stimulation of inhibitory nerves and is mediated, in part, by nitric oxide and vasoactive intestinal peptide.",
author = "Jorge Nieto and Rakestraw, {Peter C.} and Snyder, {Jack R.} and Vatistas, {Nick J.}",
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language = "English (US)",
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pages = "413--419",
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TY - JOUR

T1 - In vitro effects of erythromycin, lidocaine, and metoclopramide on smooth muscle from the pyloric antrum, proximal portion of the duodenum, and middle portion of the jejunum of horses

AU - Nieto, Jorge

AU - Rakestraw, Peter C.

AU - Snyder, Jack R.

AU - Vatistas, Nick J.

PY - 2000/4

Y1 - 2000/4

N2 - Objective - To evaluate effects of erythromycin, lidocaine, and metoclopramide on smooth muscle of the pyloric antrum (PA), proximal portion of the duodenum (PD), and middle portion of the jejunum (MJ) of horses. Sample Population - Strips of smooth muscle from 7 horses. Procedure - Isolated muscle strips were suspended in a bath and attached to isometric force transducers. Once stable spontaneous contractions were observed, agents were added. Isometric stress responses were compared with the amplitude of spontaneous contractions. Results - A single dose of erythromycin to the PA increased contractile amplitude (CA) for the longitudinal smooth muscle (mean ± SEM, 76 ± 16 g/cm2) but decreased CA for circular smooth muscle (-79 ± 23 g/cm2). The inhibitory effect was decreased by tetrodotoxin, N(G)-nitro- L-arginine methyl ester, and a vasoactive intestinal peptide antagonist. Erythromycin increased CA for the MJ, which was maximal at 10-4M (171 ± 36 g/cm2). Lidocaine increased CA for the PD, which was maximal at 10-4M (60 ± 5 g/cm2). Metoclopramide increased the CA, which was maximal at 10-4M for the PA (75 ± 26 g/cm2), PD (279 ± 33 g/cm2), and MJ (456 ± 59 g/cm2). Conclusions - Regional differences in responses to erythromycin, lidocaine, and metoclopramide were evident in the gastrointestinal tract of horses. Metoclopramide increased CA in all tissues used, whereas erythromycin inhibited CA in circular smooth muscle but stimulated CA in longitudinal smooth muscle from the PA. Inhibition is caused by stimulation of inhibitory nerves and is mediated, in part, by nitric oxide and vasoactive intestinal peptide.

AB - Objective - To evaluate effects of erythromycin, lidocaine, and metoclopramide on smooth muscle of the pyloric antrum (PA), proximal portion of the duodenum (PD), and middle portion of the jejunum (MJ) of horses. Sample Population - Strips of smooth muscle from 7 horses. Procedure - Isolated muscle strips were suspended in a bath and attached to isometric force transducers. Once stable spontaneous contractions were observed, agents were added. Isometric stress responses were compared with the amplitude of spontaneous contractions. Results - A single dose of erythromycin to the PA increased contractile amplitude (CA) for the longitudinal smooth muscle (mean ± SEM, 76 ± 16 g/cm2) but decreased CA for circular smooth muscle (-79 ± 23 g/cm2). The inhibitory effect was decreased by tetrodotoxin, N(G)-nitro- L-arginine methyl ester, and a vasoactive intestinal peptide antagonist. Erythromycin increased CA for the MJ, which was maximal at 10-4M (171 ± 36 g/cm2). Lidocaine increased CA for the PD, which was maximal at 10-4M (60 ± 5 g/cm2). Metoclopramide increased the CA, which was maximal at 10-4M for the PA (75 ± 26 g/cm2), PD (279 ± 33 g/cm2), and MJ (456 ± 59 g/cm2). Conclusions - Regional differences in responses to erythromycin, lidocaine, and metoclopramide were evident in the gastrointestinal tract of horses. Metoclopramide increased CA in all tissues used, whereas erythromycin inhibited CA in circular smooth muscle but stimulated CA in longitudinal smooth muscle from the PA. Inhibition is caused by stimulation of inhibitory nerves and is mediated, in part, by nitric oxide and vasoactive intestinal peptide.

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M3 - Article

VL - 61

SP - 413

EP - 419

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

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