In vitro differentiation of neonatal foreskin fibroblasts to chondrocytes

M. M. French, E. Liu, K. A. Athanasiou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Successful tissue engineering of articular cartilage is hampered by the lack of an abundant source of chondrocytic cells. To determine if differentiated cells can be "re-differentiated" to a chondrocytic phenotype, neonatal fibroblastic foreskin cells were cultured in monolayers on wells coated with either the cartilage proteoglycan aggrecan in PBS, or with PBS alone. Cells were cultured for four weeks, with weekly data collection points. Cells were examined for mRNA expression of collagen type II and aggrecan, matrix protein expression by Safranin O staining and Col II protein expression by immunohistochemistry. Results show that cells cultured on aggrecan initiate mRNA expression of the matrix proteins by one week. Safranin O staining is also positive, although Col II protein levels are low. At two weeks, cells maintain the mRNA expression and Safranin O staining and the Col II protein levels are seen to increase. At the three and four week periods, the message and protein levels appear stable, while the morphology of the cells changes somewhat. Smaller aggregates are either incorporated into larger existing aggregates, increase their attachments to the culture surface or die. The physiologically relevant levels of Col II message and protein can be determined by comparison with native tissue.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages785-786
Number of pages2
Volume1
StatePublished - 2002
Externally publishedYes
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

Keywords

  • Differentiation
  • Fibroblasts
  • Matrix

ASJC Scopus subject areas

  • Bioengineering

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