In vitro differentiation and propagation of urothelium from pluripotent stem cell lines

Stephanie L. Osborn, Eric A Kurzrock

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages137-144
Number of pages8
Volume1655
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1655
ISSN (Print)1064-3745

Keywords

  • Activin A
  • Bladder bioengineering
  • Definitive endoderm
  • Human embryonic stem cells
  • Induced pluripotent stem cells
  • Uroplakins
  • Urothelium

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Osborn, S. L., & Kurzrock, E. A. (2018). In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. In Methods in Molecular Biology (Vol. 1655, pp. 137-144). (Methods in Molecular Biology; Vol. 1655). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7234-0_11