Abstract
Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 137-144 |
| Number of pages | 8 |
| Volume | 1655 |
| DOIs | |
| State | Published - 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1655 |
| ISSN (Print) | 1064-3745 |
Keywords
- Activin A
- Bladder bioengineering
- Definitive endoderm
- Human embryonic stem cells
- Induced pluripotent stem cells
- Uroplakins
- Urothelium
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Osborn, S. L., & Kurzrock, E. A. (2018). In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. In Methods in Molecular Biology (Vol. 1655, pp. 137-144). (Methods in Molecular Biology; Vol. 1655). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7234-0_11