In vitro differentiation and propagation of urothelium from pluripotent stem cell lines

Stephanie L. Osborn, Eric A Kurzrock

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages137-144
Number of pages8
Volume1655
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1655
ISSN (Print)1064-3745

Fingerprint

Urothelium
Pluripotent Stem Cells
Cell Line
Urinary Bladder Diseases
Induced Pluripotent Stem Cells
Bioengineering
Culture Techniques
Endoderm
Urinary Bladder
Transplants
In Vitro Techniques
Human Embryonic Stem Cells

Keywords

  • Activin A
  • Bladder bioengineering
  • Definitive endoderm
  • Human embryonic stem cells
  • Induced pluripotent stem cells
  • Uroplakins
  • Urothelium

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Osborn, S. L., & Kurzrock, E. A. (2018). In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. In Methods in Molecular Biology (Vol. 1655, pp. 137-144). (Methods in Molecular Biology; Vol. 1655). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7234-0_11

In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. / Osborn, Stephanie L.; Kurzrock, Eric A.

Methods in Molecular Biology. Vol. 1655 Humana Press Inc., 2018. p. 137-144 (Methods in Molecular Biology; Vol. 1655).

Research output: Chapter in Book/Report/Conference proceedingChapter

Osborn, SL & Kurzrock, EA 2018, In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. in Methods in Molecular Biology. vol. 1655, Methods in Molecular Biology, vol. 1655, Humana Press Inc., pp. 137-144. https://doi.org/10.1007/978-1-4939-7234-0_11
Osborn SL, Kurzrock EA. In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. In Methods in Molecular Biology. Vol. 1655. Humana Press Inc. 2018. p. 137-144. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7234-0_11
Osborn, Stephanie L. ; Kurzrock, Eric A. / In vitro differentiation and propagation of urothelium from pluripotent stem cell lines. Methods in Molecular Biology. Vol. 1655 Humana Press Inc., 2018. pp. 137-144 (Methods in Molecular Biology).
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