In vitro differentiation and propagation of urothelium from pluripotent stem cell lines

Stephanie L. Osborn; Eric A Kurzrock

doi:10.1007/978-1-4939-7234-0_11

In vitro differentiation and propagation of urothelium from pluripotent stem cell lines

Stephanie L. Osborn, Eric A Kurzrock

Urology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	137-144
Number of pages	8
Volume	1655
DOIs	https://doi.org/10.1007/978-1-4939-7234-0_11
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1655
ISSN (Print)	1064-3745

Keywords

Activin A
Bladder bioengineering
Definitive endoderm
Human embryonic stem cells
Induced pluripotent stem cells
Uroplakins
Urothelium

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-7234-0_11

Cite this

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title = "In vitro differentiation and propagation of urothelium from pluripotent stem cell lines",

abstract = "Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.",

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AU - Kurzrock, Eric A

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AB - Bioengineering of bladder tissue, particularly for those patients who have advanced bladder disease, requires a source of urothelium that is healthy, capable of significant proliferation in vitro and immunologically tolerated upon transplant. As pluripotent stem cells have the potential to fulfill such criteria, they provide a critical cell source from which urothelium might be derived in vitro and used clinically. Herein, we describe the in vitro differentiation of urothelium from the H9 human embryonic stem cell (hESC) line through the definitive endoderm (DE) phase via selective culture techniques. The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells.

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KW - Human embryonic stem cells

KW - Induced pluripotent stem cells

KW - Uroplakins

KW - Urothelium

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In vitro differentiation and propagation of urothelium from pluripotent stem cell lines

Abstract

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Keywords

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