In vitro comparison of the effects of two forms of hydroxyethyl starch solutions on platelet function in dogs

Janelle R. Wierenga, Karl Jandrey, Steve C. Haskins, Fern Tablin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Objective - To evaluate the effect of 2 hydroxyethyl starch (HES) preparations (ie, HES solution with a molecular weight of 600 kd and a degree of substitution of 0.7 [HES 600/0.7] and a calcium-containing polyionic HES solution with a molecular weight of 670 kd and a degree of substitution of 0.75 [HES 670/0.75]) on canine platelet function. Sample Population - Blood samples from 10 healthy adult dogs. Procedures - Dilution of citrated whole blood was performed with saline (0.9% NaCl) solution, HES 600/0.7, and HES 670/0.75 at ratios of 1:9 (ie, 1 part saline solution or colloid to 9 parts whole blood) and 1:3. Measurements of time to platelet plug formation in a capillary tube (ie, closure time) were made by use of a bench-top platelet function analyzer with collagen and ADP platelet agonists. Results - Mean baseline closure time was 68.0 ± 15.3 seconds. A 1:3 dilution of whole blood with saline solution, HES 600/0.7, and HES 670/0.75 resulted in mean closure times of 85.8 ± 15.7 seconds, 100.6 ± 18.6 seconds, and 101.6 ± 16.2 seconds, respectively. Closure time following 1:3 dilution of whole blood with saline solution was significantly different from baseline and from 1:9 dilution with saline solution. Closure time following 1:3 dilution of whole blood with HES 670/0.75 was significantly different from baseline, 1:3 and 1:9 dilutions with saline solution, and 1:9 dilutions with HES 600/0.7 or HES 670/0.75. Conclusions and Clinical Relevance - Saline solution, HES 600/0.7, and HES 670/0.75 affect canine platelet function by prolonging closure times; HES solutions prolonged closure time to a greater extent than saline solution.

Original languageEnglish (US)
Pages (from-to)605-609
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume68
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

Starch
Blood Platelets
starch
Dogs
dogs
sodium chloride
Sodium Chloride
blood
In Vitro Techniques
Canidae
Molecular Weight
Hydroxyethyl Starch Derivatives
molecular weight
Colloids
colloids
Adenosine Diphosphate
agonists
collagen
Collagen
Calcium

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro comparison of the effects of two forms of hydroxyethyl starch solutions on platelet function in dogs. / Wierenga, Janelle R.; Jandrey, Karl; Haskins, Steve C.; Tablin, Fern.

In: American Journal of Veterinary Research, Vol. 68, No. 6, 06.2007, p. 605-609.

Research output: Contribution to journalArticle

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abstract = "Objective - To evaluate the effect of 2 hydroxyethyl starch (HES) preparations (ie, HES solution with a molecular weight of 600 kd and a degree of substitution of 0.7 [HES 600/0.7] and a calcium-containing polyionic HES solution with a molecular weight of 670 kd and a degree of substitution of 0.75 [HES 670/0.75]) on canine platelet function. Sample Population - Blood samples from 10 healthy adult dogs. Procedures - Dilution of citrated whole blood was performed with saline (0.9{\%} NaCl) solution, HES 600/0.7, and HES 670/0.75 at ratios of 1:9 (ie, 1 part saline solution or colloid to 9 parts whole blood) and 1:3. Measurements of time to platelet plug formation in a capillary tube (ie, closure time) were made by use of a bench-top platelet function analyzer with collagen and ADP platelet agonists. Results - Mean baseline closure time was 68.0 ± 15.3 seconds. A 1:3 dilution of whole blood with saline solution, HES 600/0.7, and HES 670/0.75 resulted in mean closure times of 85.8 ± 15.7 seconds, 100.6 ± 18.6 seconds, and 101.6 ± 16.2 seconds, respectively. Closure time following 1:3 dilution of whole blood with saline solution was significantly different from baseline and from 1:9 dilution with saline solution. Closure time following 1:3 dilution of whole blood with HES 670/0.75 was significantly different from baseline, 1:3 and 1:9 dilutions with saline solution, and 1:9 dilutions with HES 600/0.7 or HES 670/0.75. Conclusions and Clinical Relevance - Saline solution, HES 600/0.7, and HES 670/0.75 affect canine platelet function by prolonging closure times; HES solutions prolonged closure time to a greater extent than saline solution.",
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