In vitro biologic activities of the antimicrobials triclocarban, its analogs, and triclosan in bioassay screens: Receptor-based bioassay screens

Ki Chang Ahn, Bin Zhao, Jiangang Chen, Gennady Cherednichenko, Enio Sanmarti, Michael S. Denison, Bill Lasley, Isaac N Pessah, Dietmar Kültz, Daniel P Y Chang, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

234 Scopus citations

Abstract

Background: Concerns have been raised about the biological and toxicologic effects of the antimicrobials triclocarban (TCC) and triclosan (TCS) in personal care products. Few studies have evaluated their biological activities in mammalian cells to assess their potential for adverse effects. Objectives: In this study, we assessed the activity of TCC, its analogs, and TCS in in vitro nuclear-receptor-responsive and calcium signaling bioassays. Materials and Methods: We determined the biological activities of the compounds in in vitro, cell-based, and nuclear-receptor-responsive bioassays for receptors for aryl hydrocarbon (AhR), estrogen (ER), androgen (AR), and ryanodine (RyR1). Results: Some carbanilide compounds, including TCC (1-10 μM), enhanced estradiol (E2)-dependent or testosterone-dependent activation of ER- and AR-responsive gene expression up to 2.5-fold but exhibited little or no agonistic activity alone. Some carbanilides and TCS exhibited weak agonistic and/or antagonistic activity in the AhR-responsive bioassay. TCS exhibited antagonistic activity in both ER- and AR-responsive bioassays. TCS (0.1-10 μM) significantly enhanced the binding of [3H]ryanodine to RyR1 and caused elevation of resting cytosolic [Ca2+] in primary skeletal myotubes, but carbanilides had no effect. Conclusions: Carbanilides, including TCC, enhanced hormone-dependent induction of ER- and AR-dependent gene expression but had little agonist activity, suggesting a new mechanism of action of endocrine-disrupting compounds. TCS, structurally similar to noncoplanar ortho-substituted polychlorinated biphenyls, exhibited weak AhR activity but interacted with RyR1 and stimulated Ca2+ mobilization. These observations have potential implications for human and animal health. Further investigations are needed into the biological and toxicologic effects of TCC, its analogs, and TCS.

Original languageEnglish (US)
Pages (from-to)1203-1210
Number of pages8
JournalEnvironmental Health Perspectives
Volume116
Issue number9
DOIs
StatePublished - Sep 2008

Keywords

  • Androgen receptor
  • Antimicrobial
  • Aryl hydrocarbon receptor
  • Bioactivity
  • Carbanilide analog
  • Estrogen receptor
  • Ryanodine receptor
  • Sensitization
  • Signal amplification
  • Triclocarbon
  • Triclosan

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

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    Ahn, K. C., Zhao, B., Chen, J., Cherednichenko, G., Sanmarti, E., Denison, M. S., Lasley, B., Pessah, I. N., Kültz, D., Chang, D. P. Y., Gee, S. J., & Hammock, B. D. (2008). In vitro biologic activities of the antimicrobials triclocarban, its analogs, and triclosan in bioassay screens: Receptor-based bioassay screens. Environmental Health Perspectives, 116(9), 1203-1210. https://doi.org/10.1289/ehp.11200