In vitro antimicrobial activity of Shiva-11 against ocular pathogens

L. Gunshefski, Mark J Mannis, James S Cullor, I. R. Schwab, J. Jaynes, W. L. Smith, E. Mabry, Christopher J Murphy

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Cecropins are antimicrobial peptides (30-35 amino acids) isolated from the hemolymph of the cecropia moth. Previous studies have demonstrated their antimicrobial efficacy against a variety of pathogens, including both gram- positive and -negative bacteria, fungi, protozoa, and enveloped viruses. To assess their therapeutic potential against ocular pathogens, we analyzed the in vitro antimicrobial activity of a synthetic cecropin analog, Shiva-11, against virulent microbial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans), Bacterial isolates were obtained from human cases of severe ulcerative keratitis. Shiva-11 was tested at varying concentrations in bacterial suspensions containing 1-2 x 106 CFU/ml at 37°C. Samples were plated on nutrient agar and colonies counted after 24-48 h of incubation. Shiva-11 yielded >3 log killing of all isolates after 60 min of exposure to this compound. The results of this study indicate that Shiva-11 possesses broad-spectrum in vitro antimicrobial activity against human clinical ocular pathogens.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalCornea
Volume13
Issue number3
StatePublished - 1994
Externally publishedYes

Keywords

  • Antimicrobial peptides
  • Cecropins
  • Ocular pathogens
  • Shiva-11

ASJC Scopus subject areas

  • Ophthalmology

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  • Cite this

    Gunshefski, L., Mannis, M. J., Cullor, J. S., Schwab, I. R., Jaynes, J., Smith, W. L., Mabry, E., & Murphy, C. J. (1994). In vitro antimicrobial activity of Shiva-11 against ocular pathogens. Cornea, 13(3), 237-242.