Abstract
Cecropins are antimicrobial peptides (30-35 amino acids) isolated from the hemolymph of the cecropia moth. Previous studies have demonstrated their antimicrobial efficacy against a variety of pathogens, including both gram- positive and -negative bacteria, fungi, protozoa, and enveloped viruses. To assess their therapeutic potential against ocular pathogens, we analyzed the in vitro antimicrobial activity of a synthetic cecropin analog, Shiva-11, against virulent microbial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans), Bacterial isolates were obtained from human cases of severe ulcerative keratitis. Shiva-11 was tested at varying concentrations in bacterial suspensions containing 1-2 x 106 CFU/ml at 37°C. Samples were plated on nutrient agar and colonies counted after 24-48 h of incubation. Shiva-11 yielded >3 log killing of all isolates after 60 min of exposure to this compound. The results of this study indicate that Shiva-11 possesses broad-spectrum in vitro antimicrobial activity against human clinical ocular pathogens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 237-242 |
| Number of pages | 6 |
| Journal | Cornea |
| Volume | 13 |
| Issue number | 3 |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- Antimicrobial peptides
- Cecropins
- Ocular pathogens
- Shiva-11
ASJC Scopus subject areas
- Ophthalmology
Cite this
In vitro antimicrobial activity of Shiva-11 against ocular pathogens. / Gunshefski, L.; Mannis, Mark J; Cullor, James S; Schwab, I. R.; Jaynes, J.; Smith, W. L.; Mabry, E.; Murphy, Christopher J.
In: Cornea, Vol. 13, No. 3, 1994, p. 237-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro antimicrobial activity of Shiva-11 against ocular pathogens
AU - Gunshefski, L.
AU - Mannis, Mark J
AU - Cullor, James S
AU - Schwab, I. R.
AU - Jaynes, J.
AU - Smith, W. L.
AU - Mabry, E.
AU - Murphy, Christopher J
PY - 1994
Y1 - 1994
N2 - Cecropins are antimicrobial peptides (30-35 amino acids) isolated from the hemolymph of the cecropia moth. Previous studies have demonstrated their antimicrobial efficacy against a variety of pathogens, including both gram- positive and -negative bacteria, fungi, protozoa, and enveloped viruses. To assess their therapeutic potential against ocular pathogens, we analyzed the in vitro antimicrobial activity of a synthetic cecropin analog, Shiva-11, against virulent microbial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans), Bacterial isolates were obtained from human cases of severe ulcerative keratitis. Shiva-11 was tested at varying concentrations in bacterial suspensions containing 1-2 x 106 CFU/ml at 37°C. Samples were plated on nutrient agar and colonies counted after 24-48 h of incubation. Shiva-11 yielded >3 log killing of all isolates after 60 min of exposure to this compound. The results of this study indicate that Shiva-11 possesses broad-spectrum in vitro antimicrobial activity against human clinical ocular pathogens.
AB - Cecropins are antimicrobial peptides (30-35 amino acids) isolated from the hemolymph of the cecropia moth. Previous studies have demonstrated their antimicrobial efficacy against a variety of pathogens, including both gram- positive and -negative bacteria, fungi, protozoa, and enveloped viruses. To assess their therapeutic potential against ocular pathogens, we analyzed the in vitro antimicrobial activity of a synthetic cecropin analog, Shiva-11, against virulent microbial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans), Bacterial isolates were obtained from human cases of severe ulcerative keratitis. Shiva-11 was tested at varying concentrations in bacterial suspensions containing 1-2 x 106 CFU/ml at 37°C. Samples were plated on nutrient agar and colonies counted after 24-48 h of incubation. Shiva-11 yielded >3 log killing of all isolates after 60 min of exposure to this compound. The results of this study indicate that Shiva-11 possesses broad-spectrum in vitro antimicrobial activity against human clinical ocular pathogens.
KW - Antimicrobial peptides
KW - Cecropins
KW - Ocular pathogens
KW - Shiva-11
UR - http://www.scopus.com/inward/record.url?scp=0028334859&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028334859&partnerID=8YFLogxK
M3 - Article
C2 - 8033574
AN - SCOPUS:0028334859
VL - 13
SP - 237
EP - 242
JO - Cornea
JF - Cornea
SN - 0277-3740
IS - 3
ER -