In vitro antimicrobial activity of Shiva-11 against ocular pathogens

L. Gunshefski, Mark J Mannis, James S Cullor, I. R. Schwab, J. Jaynes, W. L. Smith, E. Mabry, Christopher J Murphy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cecropins are antimicrobial peptides (30-35 amino acids) isolated from the hemolymph of the cecropia moth. Previous studies have demonstrated their antimicrobial efficacy against a variety of pathogens, including both gram- positive and -negative bacteria, fungi, protozoa, and enveloped viruses. To assess their therapeutic potential against ocular pathogens, we analyzed the in vitro antimicrobial activity of a synthetic cecropin analog, Shiva-11, against virulent microbial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans), Bacterial isolates were obtained from human cases of severe ulcerative keratitis. Shiva-11 was tested at varying concentrations in bacterial suspensions containing 1-2 x 106 CFU/ml at 37°C. Samples were plated on nutrient agar and colonies counted after 24-48 h of incubation. Shiva-11 yielded >3 log killing of all isolates after 60 min of exposure to this compound. The results of this study indicate that Shiva-11 possesses broad-spectrum in vitro antimicrobial activity against human clinical ocular pathogens.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalCornea
Volume13
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Cecropins
Staphylococcal Pneumonia
Corneal Ulcer
Hemolymph
Moths
Gram-Positive Bacteria
Streptococcus pneumoniae
Gram-Negative Bacteria
Candida albicans
Human Activities
Pseudomonas aeruginosa
Agar
Suspensions
Fungi
Viruses
Amino Acids
Food
Peptides
In Vitro Techniques
Shiva 11

Keywords

  • Antimicrobial peptides
  • Cecropins
  • Ocular pathogens
  • Shiva-11

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Gunshefski, L., Mannis, M. J., Cullor, J. S., Schwab, I. R., Jaynes, J., Smith, W. L., ... Murphy, C. J. (1994). In vitro antimicrobial activity of Shiva-11 against ocular pathogens. Cornea, 13(3), 237-242.

In vitro antimicrobial activity of Shiva-11 against ocular pathogens. / Gunshefski, L.; Mannis, Mark J; Cullor, James S; Schwab, I. R.; Jaynes, J.; Smith, W. L.; Mabry, E.; Murphy, Christopher J.

In: Cornea, Vol. 13, No. 3, 1994, p. 237-242.

Research output: Contribution to journalArticle

Gunshefski, L, Mannis, MJ, Cullor, JS, Schwab, IR, Jaynes, J, Smith, WL, Mabry, E & Murphy, CJ 1994, 'In vitro antimicrobial activity of Shiva-11 against ocular pathogens', Cornea, vol. 13, no. 3, pp. 237-242.
Gunshefski L, Mannis MJ, Cullor JS, Schwab IR, Jaynes J, Smith WL et al. In vitro antimicrobial activity of Shiva-11 against ocular pathogens. Cornea. 1994;13(3):237-242.
Gunshefski, L. ; Mannis, Mark J ; Cullor, James S ; Schwab, I. R. ; Jaynes, J. ; Smith, W. L. ; Mabry, E. ; Murphy, Christopher J. / In vitro antimicrobial activity of Shiva-11 against ocular pathogens. In: Cornea. 1994 ; Vol. 13, No. 3. pp. 237-242.
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