In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus

Duane A. Robinson, Ronald W. Griffith, Dan Shechtman, Richard B. Evans, Michael G. Conzemius

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Bacterial infections are a costly sequela in any wound. The corrosion properties of 0.15, 0.30, 0.45 and 0.60 g of Mg metal were determined in Mueller-Hinton broth by serially measuring the Mg2+ concentrations and pH over 72 h. In addition, the effect of Mg metal, increased Mg2+ concentration and alkaline pH on the in vitro growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were evaluated in three separate experiments. The primary outcome measure for culture studies was colony-forming units/ml compared to appropriate positive and/or negative controls. Regardless of the mass of Mg added, there was a predictable increase in pH and Mg2+ concentration. The addition of Mg and an increase of pH resulted in antibacterial effects similar to the fluoroquinolone antibiotic; however, a simple increase in Mg2+ concentration alone had no effect. The results demonstrate an antibacterial effect of Mg on three common aerobic bacterial organisms, the mechanism of which appears to be an alkaline pH.

Original languageEnglish (US)
Pages (from-to)1869-1877
Number of pages9
JournalActa Biomaterialia
Volume6
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

Pseudomonas aeruginosa
Magnesium
Escherichia coli
Staphylococcus aureus
Metals
Fluoroquinolones
Antibiotics
Corrosion
Anti-Bacterial Agents
Bacterial Infections
Experiments
Stem Cells
Outcome Assessment (Health Care)
In Vitro Techniques
Wounds and Injuries
Growth

Keywords

  • Antimicrobial
  • Infection
  • Magnesium
  • Metal

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. / Robinson, Duane A.; Griffith, Ronald W.; Shechtman, Dan; Evans, Richard B.; Conzemius, Michael G.

In: Acta Biomaterialia, Vol. 6, No. 5, 05.2010, p. 1869-1877.

Research output: Contribution to journalArticle

Robinson, Duane A. ; Griffith, Ronald W. ; Shechtman, Dan ; Evans, Richard B. ; Conzemius, Michael G. / In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In: Acta Biomaterialia. 2010 ; Vol. 6, No. 5. pp. 1869-1877.
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