TY - JOUR
T1 - In vitro and in vivo toxicity of urban and rural particulate matter from California
AU - Mirowsky, Jaime E.
AU - Jin, Lan
AU - Thurston, George
AU - Lighthall, David
AU - Tyner, Tim
AU - Horton, Lori
AU - Galdanes, Karen
AU - Chillrud, Steven
AU - Ross, James
AU - Pinkerton, Kent E
AU - Chen, Lung Chi
AU - Lippmann, Morton
AU - Gordon, Terry
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Particulate matter (PM) varies in chemical composition and mass concentration based on location, source, and particle size. This study sought to evaluate the invitro and invivo toxicity of coarse (PM10-2.5) and fine (PM2.5) PM samples collected at 5 diverse sites within California. Coarse and fine PM samples were collected simultaneously at 2 rural and 3 urban sites within California during the summer. A human pulmonary microvascular endothelial cell line (HPMEC-ST1.6R) was exposed to PM suspensions (50μg/mL) and analyzed for reactive oxygen species (ROS) after 5h of treatment. In addition, FVB/N mice were exposed by oropharyngeal aspiration to 50μg PM, and lavage fluid was collected 24h post-exposure and analyzed for total protein and %PMNs. Correlations between trace metal concentrations, endotoxin, and biological endpoints were calculated, and the effect of particle size range, locale (urban vs. rural), and location was determined. Absolute principal factor analysis was used to identify pollution sources of PM from elemental tracers of those sources. Ambient PM elicited an ROS and pro-inflammatory-related response in the cell and mouse models, respectively. These responses were dependent on particle size, locale, and location. Trace elements associated with soil and traffic markers were most strongly linked to the adverse effects invitro and invivo. Particle size, location, source, and composition of PM collected at 5 locations in California affected the ROS response in human pulmonary endothelial cells and the inflammatory response in mice.
AB - Particulate matter (PM) varies in chemical composition and mass concentration based on location, source, and particle size. This study sought to evaluate the invitro and invivo toxicity of coarse (PM10-2.5) and fine (PM2.5) PM samples collected at 5 diverse sites within California. Coarse and fine PM samples were collected simultaneously at 2 rural and 3 urban sites within California during the summer. A human pulmonary microvascular endothelial cell line (HPMEC-ST1.6R) was exposed to PM suspensions (50μg/mL) and analyzed for reactive oxygen species (ROS) after 5h of treatment. In addition, FVB/N mice were exposed by oropharyngeal aspiration to 50μg PM, and lavage fluid was collected 24h post-exposure and analyzed for total protein and %PMNs. Correlations between trace metal concentrations, endotoxin, and biological endpoints were calculated, and the effect of particle size range, locale (urban vs. rural), and location was determined. Absolute principal factor analysis was used to identify pollution sources of PM from elemental tracers of those sources. Ambient PM elicited an ROS and pro-inflammatory-related response in the cell and mouse models, respectively. These responses were dependent on particle size, locale, and location. Trace elements associated with soil and traffic markers were most strongly linked to the adverse effects invitro and invivo. Particle size, location, source, and composition of PM collected at 5 locations in California affected the ROS response in human pulmonary endothelial cells and the inflammatory response in mice.
KW - Aspiration exposure
KW - Inflammation
KW - Invitro exposure
KW - Particulate matter
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84922949431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922949431&partnerID=8YFLogxK
U2 - 10.1016/j.atmosenv.2014.12.051
DO - 10.1016/j.atmosenv.2014.12.051
M3 - Article
AN - SCOPUS:84922949431
VL - 103
SP - 256
EP - 262
JO - Atmospheric Environment
JF - Atmospheric Environment
SN - 1352-2310
ER -