In vitro and in vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies

Edward B. Garon, David Sawcer, P. Thomas Vernier, Tao Tang, Yinghua Sun, Laura Marcu, Martin A. Gundersen, H. Phillip Koeffler

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

When delivered to cells, very short duration, high electric field pulses (nanoelectropulses) induce primarily intracellular events. We present evidence that this emerging modality may have a role as a local cancer therapy. Five hematologic and 16 solid tumor cell lines were pulsed in vitro. Hematologic cells proved particularly sensitive to nanoelectropulses, with more than a 60% decrease in viable cells measured by MTT assay 96 hr after pulsing in 4 of 5 cell lines. In solid tumor cell lines, 10 out of 16 cell lines had more than a 10% decrease in viable cells. AsPC-1, a pancreatic cancer cell line, demonstrated the greatest in vitro sensitivity among solid tumor cell lines, with a 64% decrease in viable cells. When nanoelectropulse therapy was applied to AsPC-1 tumors in athymic nude mice, responses were seen in 4 of 6 tumors, including clinical complete responses in 3 of 6 animals. A single human subject applied nanoelectropulse therapy to his own basal cell carcinoma and had a complete pathologic response. In summary, we demonstrate that electric pulses 20 ns or less kill a wide variety of human cancer cells in vitro, induce tumor regression in vivo, and show efficacy in a single human patient. Therefore, nanoelectropulse therapy deserves further study as a potentially effective cancer therapy.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalInternational Journal of Cancer
Volume121
Issue number3
DOIs
StatePublished - Aug 1 2007

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Tumor Cell Line
Neoplasms
Nude Mice
Cell Line
Therapeutics
Basal Cell Carcinoma
Pancreatic Neoplasms
In Vitro Techniques

Keywords

  • Basal cell carcinoma
  • Nanoelectropulse therapy
  • Pancreatic cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro and in vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies. / Garon, Edward B.; Sawcer, David; Vernier, P. Thomas; Tang, Tao; Sun, Yinghua; Marcu, Laura; Gundersen, Martin A.; Koeffler, H. Phillip.

In: International Journal of Cancer, Vol. 121, No. 3, 01.08.2007, p. 675-682.

Research output: Contribution to journalArticle

Garon, EB, Sawcer, D, Vernier, PT, Tang, T, Sun, Y, Marcu, L, Gundersen, MA & Koeffler, HP 2007, 'In vitro and in vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies', International Journal of Cancer, vol. 121, no. 3, pp. 675-682. https://doi.org/10.1002/ijc.22723
Garon, Edward B. ; Sawcer, David ; Vernier, P. Thomas ; Tang, Tao ; Sun, Yinghua ; Marcu, Laura ; Gundersen, Martin A. ; Koeffler, H. Phillip. / In vitro and in vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies. In: International Journal of Cancer. 2007 ; Vol. 121, No. 3. pp. 675-682.
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