In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells

Bosco M C Chan, Nariaki Matsuura, Yoshikazu Takada, Bruce R. Zetter, Martin E. Hemler

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

Cloned integrin α2 subunit complementary DNA was expressed on human rhabdomyosarcoma (RD) cells to give a functional VLA-2 (α2β1) adhesion receptor. The VLA-2-positive RDA2 cells not only showed increased adhesion to collagen and laminin in vitro, but also formed substantially more metastatic tumor colonies in nude mice after either intravenous or subcutaneous injection. These results show that a specific adhesion receptor (VLA-2) can markedly enhance both experimental and spontaneous metastasis. In contrast to the metastasis results, there was no difference in either the in vitro growth rate or apparent in vivo tumorigenicity of RD and RDA2 cells.

Original languageEnglish (US)
Pages (from-to)1600-1602
Number of pages3
JournalScience
Volume251
Issue number5001
StatePublished - Mar 29 1991
Externally publishedYes

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Integrin alpha2beta1
Rhabdomyosarcoma
Neoplasm Metastasis
Laminin
Subcutaneous Injections
Nude Mice
Integrins
Intravenous Injections
Collagen
Complementary DNA
Growth
In Vitro Techniques
Neoplasms
adhesion receptor

ASJC Scopus subject areas

  • General

Cite this

Chan, B. M. C., Matsuura, N., Takada, Y., Zetter, B. R., & Hemler, M. E. (1991). In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. Science, 251(5001), 1600-1602.

In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. / Chan, Bosco M C; Matsuura, Nariaki; Takada, Yoshikazu; Zetter, Bruce R.; Hemler, Martin E.

In: Science, Vol. 251, No. 5001, 29.03.1991, p. 1600-1602.

Research output: Contribution to journalArticle

Chan, BMC, Matsuura, N, Takada, Y, Zetter, BR & Hemler, ME 1991, 'In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells', Science, vol. 251, no. 5001, pp. 1600-1602.
Chan BMC, Matsuura N, Takada Y, Zetter BR, Hemler ME. In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. Science. 1991 Mar 29;251(5001):1600-1602.
Chan, Bosco M C ; Matsuura, Nariaki ; Takada, Yoshikazu ; Zetter, Bruce R. ; Hemler, Martin E. / In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. In: Science. 1991 ; Vol. 251, No. 5001. pp. 1600-1602.
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