In vitro activity of acyclic nucleoside phosphonate derivatives against feline immunodeficiency virus in Crandell feline kidney cell s and feline peripheral blood lymphocytes

K. Hartmann, J. Balzarini, J. Higgins, E. De Clercq, Niels C Pedersen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Several novel fluorinated acyclic nucleoside phosphonates [i.e. 9-(3-fluoro-2-phosphonylmethoxy propyl)adenine (FPMPA) and 9-(3-fluoro-2-phosphonylmethoxypropyl)-2,6-diaminopurine (FPMP-DAP)] were evaluated for their inhibitory effect against feline immunodeficiency virus (FIV) replication in Crandell feline kidney (CrFK) cells and feline peripheral blood lymphocytes (PBL) in vitro. Whereas 3'-azido-3'deoxythymidine (AZT) was not able to achieve complete suppression of viral antigen expression and reverse transcriptase activity in the FIV-infected cell culture supernatants at 25 μM, FPMPA, FPMPDAP, and the 9-(2-phosphonylmethoxyethyl)purine derivatives PMEA and PMEDAP fully protected FIV-infected cells at 5 μM. Both FPMPA and FPMPDAP were endowed with a higher antiviral potency and/or therapeutic selectivity than PMEA and PMEDAP in inhibiting FIV infection, mainly due to a markedly lower toxicity for the cell cultures.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalAntiviral Chemistry and Chemotherapy
Volume5
Issue number1
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Virology
  • Pharmacology

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