IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS

MichaelR Harrison, TimothyM Crombleholme, Alice F Tarantal, R. Nathan Slotnick, MitchellS Golbus, EsmailD Zanjani

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

To evaluate the potential of in-utero transplantation of fetal haemopoietic stem cells (HSCs) for permanent engraftment as a treatment of congenital haemoglobinopathies, fetal rhesus monkeys were transplanted with HSCs derived from fetal livers. Five pregnant monkeys (60-62 days' gestation) were given an in-utero intraperitoneal injection of fetal liver cells (108-109 cells/kg estimated fetal recipient body weight) derived from opposite sex donors at 59-68 days' gestation. Engraftment was confirmed by karyotype analysis of peripheral blood leucocytes and bone marrow; cells of donor sex were found among the recipient cells. Donor cell engraftment was apparent in four of five in-utero HSC transplant recipients at birth. Engraftment involved lymphoid (2·9-8·0% donor cells), erythroid (5·3-12·5%), and myeloid (8·5-15·4%) lineages and has persisted for up to 2 years without evidence of graft-versus-host disease.

Original languageEnglish (US)
Pages (from-to)1425-1427
Number of pages3
JournalThe Lancet
Volume334
Issue number8677
DOIs
StatePublished - Dec 16 1989

ASJC Scopus subject areas

  • Medicine(all)

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    Harrison, M., Crombleholme, T., Tarantal, A. F., Nathan Slotnick, R., Golbus, M., & Zanjani, E. (1989). IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS. The Lancet, 334(8677), 1425-1427. https://doi.org/10.1016/S0140-6736(89)92036-9