IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS

MichaelR Harrison, TimothyM Crombleholme, Alice F Tarantal, R. Nathan Slotnick, MitchellS Golbus, EsmailD Zanjani

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

To evaluate the potential of in-utero transplantation of fetal haemopoietic stem cells (HSCs) for permanent engraftment as a treatment of congenital haemoglobinopathies, fetal rhesus monkeys were transplanted with HSCs derived from fetal livers. Five pregnant monkeys (60-62 days' gestation) were given an in-utero intraperitoneal injection of fetal liver cells (108-109 cells/kg estimated fetal recipient body weight) derived from opposite sex donors at 59-68 days' gestation. Engraftment was confirmed by karyotype analysis of peripheral blood leucocytes and bone marrow; cells of donor sex were found among the recipient cells. Donor cell engraftment was apparent in four of five in-utero HSC transplant recipients at birth. Engraftment involved lymphoid (2·9-8·0% donor cells), erythroid (5·3-12·5%), and myeloid (8·5-15·4%) lineages and has persisted for up to 2 years without evidence of graft-versus-host disease.

Original languageEnglish (US)
Pages (from-to)1425-1427
Number of pages3
JournalThe Lancet
Volume334
Issue number8677
DOIs
StatePublished - Dec 16 1989

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Liver Transplantation
Haplorhini
Stem Cells
Fetal Stem Cells
Tissue Donors
Hemoglobinopathies
Pregnancy
Erythroid Cells
Fetal Weight
Liver
Graft vs Host Disease
Intraperitoneal Injections
Macaca mulatta
Karyotype
Leukocytes
Transplantation
Bone Marrow
Parturition
Transplants
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Harrison, M., Crombleholme, T., Tarantal, A. F., Nathan Slotnick, R., Golbus, M., & Zanjani, E. (1989). IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS. The Lancet, 334(8677), 1425-1427. https://doi.org/10.1016/S0140-6736(89)92036-9

IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS. / Harrison, MichaelR; Crombleholme, TimothyM; Tarantal, Alice F; Nathan Slotnick, R.; Golbus, MitchellS; Zanjani, EsmailD.

In: The Lancet, Vol. 334, No. 8677, 16.12.1989, p. 1425-1427.

Research output: Contribution to journalArticle

Harrison, M, Crombleholme, T, Tarantal, AF, Nathan Slotnick, R, Golbus, M & Zanjani, E 1989, 'IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS', The Lancet, vol. 334, no. 8677, pp. 1425-1427. https://doi.org/10.1016/S0140-6736(89)92036-9
Harrison M, Crombleholme T, Tarantal AF, Nathan Slotnick R, Golbus M, Zanjani E. IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS. The Lancet. 1989 Dec 16;334(8677):1425-1427. https://doi.org/10.1016/S0140-6736(89)92036-9
Harrison, MichaelR ; Crombleholme, TimothyM ; Tarantal, Alice F ; Nathan Slotnick, R. ; Golbus, MitchellS ; Zanjani, EsmailD. / IN-UTHRO TRANSPLANTATION OF FETAL LIVER HAEMOPOIETIC STEM CELLS IN MONKEYS. In: The Lancet. 1989 ; Vol. 334, No. 8677. pp. 1425-1427.
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