In utero hematopoietic stem cell transplants prolong survival of postnatal kidney transplantation in monkeys

George B. Mychaliska, Henry E. Rice, Alice F Tarantal, Peter G. Stock, Jan Capper, Marvin R. Garovoy, Jean L. Olson, Morton J. Cowan, Michael R. Harrison

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The authors hypothesized that in utero transplantation of T-cell- depleted paternal marrow into rhesus monkey fetuses would induce tolerance to postnatal kidney grafts from the marrow donor. T-cell-depleted paternal bone marrow was transplanted intraperitoneally into two female fetal rhesus monkeys at 61 ± 1 days' gestation. Chimeric monkeys (n = 2) received kidney transplants from paternal donors. Control monkeys (n = 2) underwent kidney transplants without prior in utero stem cell transplants. Both chimeric monkeys demonstrated low level (<0.1% donor cells) engraftment in the bone marrow and peripheral blood using the polymerase chain reaction assay for the Y chromosome. The mixed lymphocyte reaction demonstrated hyporeactivity to the donor. Control animals demonstrated severe acute rejection and graft failure 1 week posttransplant. The first chimeric monkey had no significant clinical or sonographic evidence of renal failure until 7 weeks after the transplant. Biopsy findings showed mild rejection 1 week postoperatively, but rejection did not significantly progress until 5 weeks later. The second chimeric monkey had no significant clinical or sonographic changes for 4 weeks, but evidence of moderate rejection was seen on biopsy results. This monkey was given a 10-week course of immunosuppression, and had no clinical or sonographic renal deterioration, although biopsy results showed chronic rejection that was confirmed when electively euthanized 8 months later. Our data suggest that in utero transplantation of hematopoietic stem cells can increase the survival of a kidney allograft in the rhesus monkey.

Original languageEnglish (US)
Pages (from-to)976-981
Number of pages6
JournalJournal of Pediatric Surgery
Volume32
Issue number7
DOIs
StatePublished - Jul 1997

Fingerprint

Hematopoietic Stem Cells
Kidney Transplantation
Haplorhini
Transplants
Kidney
Bone Marrow
Macaca mulatta
Biopsy
T-Lymphocytes
Mixed Lymphocyte Culture Test
Y Chromosome
Hematopoietic Stem Cell Transplantation
Graft Rejection
Immunosuppression
Renal Insufficiency
Allografts
Fetus
Stem Cells
Transplantation
Pregnancy

Keywords

  • In utero hematopoietic stem cell transplantation
  • Kidney transplantation
  • Tolerance induction

ASJC Scopus subject areas

  • Surgery

Cite this

In utero hematopoietic stem cell transplants prolong survival of postnatal kidney transplantation in monkeys. / Mychaliska, George B.; Rice, Henry E.; Tarantal, Alice F; Stock, Peter G.; Capper, Jan; Garovoy, Marvin R.; Olson, Jean L.; Cowan, Morton J.; Harrison, Michael R.

In: Journal of Pediatric Surgery, Vol. 32, No. 7, 07.1997, p. 976-981.

Research output: Contribution to journalArticle

Mychaliska, GB, Rice, HE, Tarantal, AF, Stock, PG, Capper, J, Garovoy, MR, Olson, JL, Cowan, MJ & Harrison, MR 1997, 'In utero hematopoietic stem cell transplants prolong survival of postnatal kidney transplantation in monkeys', Journal of Pediatric Surgery, vol. 32, no. 7, pp. 976-981. https://doi.org/10.1016/S0022-3468(97)90381-X
Mychaliska, George B. ; Rice, Henry E. ; Tarantal, Alice F ; Stock, Peter G. ; Capper, Jan ; Garovoy, Marvin R. ; Olson, Jean L. ; Cowan, Morton J. ; Harrison, Michael R. / In utero hematopoietic stem cell transplants prolong survival of postnatal kidney transplantation in monkeys. In: Journal of Pediatric Surgery. 1997 ; Vol. 32, No. 7. pp. 976-981.
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