In situ localization of tenascin mRNA in developing mouse teeth

Richard P Tucker, J. R D Moiseiwitsch, J. M. Lauder

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tenascin is a large extracellular-matrix glycoprotein found in developing connective tissue. A cDNA probe to mouse tenascin, mTN2, was used to determine the cellular origins of this molecule in the murine tooth germ by in situ hybridization. At embryonic day 19, a hybridization signal significantly greater than background was detected with mTN2 in the subodontoblastic layer of the dental mesenchyme and in the inner enamel epithelium of the enamel organ. At postnatal day 1, a signal was detected over pre-odontoblasts and the strata intermedium and externum. No tenascin mRNA was detected in odontoblasts or the stellate reticulum at either age, and hybridization in ameloblasts was not significantly greater than background at postnatal day 1. Thus, much of the tenascin found throughout developing teeth appears to be synthesized by pre-odontoblasts and the inner enamel epithelium, the two populations of cells destined to generate mineralized matrix.

Original languageEnglish (US)
Pages (from-to)1025-1029
Number of pages5
JournalArchives of Oral Biology
Volume38
Issue number12
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Tenascin
Odontoblasts
Tooth
Messenger RNA
Dental Enamel
Epithelium
Enamel Organ
Tooth Germ
Ameloblasts
Reticulum
Mesoderm
Connective Tissue
In Situ Hybridization
Extracellular Matrix
Glycoproteins
Complementary DNA
Population

Keywords

  • cytotactin
  • extracellular matrix
  • odontogenesis
  • tenascin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

In situ localization of tenascin mRNA in developing mouse teeth. / Tucker, Richard P; Moiseiwitsch, J. R D; Lauder, J. M.

In: Archives of Oral Biology, Vol. 38, No. 12, 1993, p. 1025-1029.

Research output: Contribution to journalArticle

Tucker, Richard P ; Moiseiwitsch, J. R D ; Lauder, J. M. / In situ localization of tenascin mRNA in developing mouse teeth. In: Archives of Oral Biology. 1993 ; Vol. 38, No. 12. pp. 1025-1029.
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