TY - JOUR
T1 - In situ localization of tenascin mRNA in developing mouse teeth
AU - Tucker, Richard P
AU - Moiseiwitsch, J. R D
AU - Lauder, J. M.
PY - 1993
Y1 - 1993
N2 - Tenascin is a large extracellular-matrix glycoprotein found in developing connective tissue. A cDNA probe to mouse tenascin, mTN2, was used to determine the cellular origins of this molecule in the murine tooth germ by in situ hybridization. At embryonic day 19, a hybridization signal significantly greater than background was detected with mTN2 in the subodontoblastic layer of the dental mesenchyme and in the inner enamel epithelium of the enamel organ. At postnatal day 1, a signal was detected over pre-odontoblasts and the strata intermedium and externum. No tenascin mRNA was detected in odontoblasts or the stellate reticulum at either age, and hybridization in ameloblasts was not significantly greater than background at postnatal day 1. Thus, much of the tenascin found throughout developing teeth appears to be synthesized by pre-odontoblasts and the inner enamel epithelium, the two populations of cells destined to generate mineralized matrix.
AB - Tenascin is a large extracellular-matrix glycoprotein found in developing connective tissue. A cDNA probe to mouse tenascin, mTN2, was used to determine the cellular origins of this molecule in the murine tooth germ by in situ hybridization. At embryonic day 19, a hybridization signal significantly greater than background was detected with mTN2 in the subodontoblastic layer of the dental mesenchyme and in the inner enamel epithelium of the enamel organ. At postnatal day 1, a signal was detected over pre-odontoblasts and the strata intermedium and externum. No tenascin mRNA was detected in odontoblasts or the stellate reticulum at either age, and hybridization in ameloblasts was not significantly greater than background at postnatal day 1. Thus, much of the tenascin found throughout developing teeth appears to be synthesized by pre-odontoblasts and the inner enamel epithelium, the two populations of cells destined to generate mineralized matrix.
KW - cytotactin
KW - extracellular matrix
KW - odontogenesis
KW - tenascin
UR - http://www.scopus.com/inward/record.url?scp=0027728321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027728321&partnerID=8YFLogxK
U2 - 10.1016/0003-9969(93)90163-G
DO - 10.1016/0003-9969(93)90163-G
M3 - Article
C2 - 7511372
AN - SCOPUS:0027728321
VL - 38
SP - 1025
EP - 1029
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 12
ER -