In silico-initiated cloning and molecular characterization of a novel human member of the L1 gene family of neural cell adhesion molecules

Ming Hui Wei, Irina Karavanova, Sergey V. Ivanov, Nicolae C. Popescu, Catherine L. Keck, Svetlana Pack, Jonathan A Eisen, Michael I. Lerman

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

To discover genes contributing to mental retardation in 3p- syndrome patients we have used in silico searches for neural genes in NCBI databases (dbEST and UniGene). An EST with strong homology to the rat CAM L1 gene subsequently mapped to 3p26 was used to isolate a full-length cDNA. Molecular analysis of this cDNA, referred to as CALL (cell adhesion L1-like), showed that it is encoded by a chromosome 3p26 locus and is a novel member of the L1 gene family of neural cell adhesion molecules. Multiple lines of evidence suggest CALL is likely the human ortholog of the murine gene CHL1: it is 84% identical on the protein level, has the same domain structure, same membrane topology, and a similar expression pattern. The orthology of CALL and CHL1 was confirmed by phylogenetic analysis. By in situ hybridization, CALL is shown to be expressed regionally in a timely fashion in the central nervous system, spinal cord, and peripheral nervous system during rat development. Northern analysis and EST representation reveal that it is expressed in the brain and also outside the nervous system in some adult human tissues and tumor cell lines. The cytoplasmic domain of CALL is conserved among other members of the L1 subfamily and features sequence motifs that may involve CALL in signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)355-364
Number of pages10
JournalHuman Genetics
Volume103
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Neural Cell Adhesion Molecules
Molecular Cloning
Cell Adhesion
Computer Simulation
Genes
Expressed Sequence Tags
Complementary DNA
Peripheral Nervous System
Tumor Cell Line
Intellectual Disability
Nervous System
In Situ Hybridization
Signal Transduction
Spinal Cord
Central Nervous System
Chromosomes
Databases
Membranes
Brain
Proteins

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Wei, M. H., Karavanova, I., Ivanov, S. V., Popescu, N. C., Keck, C. L., Pack, S., ... Lerman, M. I. (1998). In silico-initiated cloning and molecular characterization of a novel human member of the L1 gene family of neural cell adhesion molecules. Human Genetics, 103(3), 355-364. https://doi.org/10.1007/s004390050829

In silico-initiated cloning and molecular characterization of a novel human member of the L1 gene family of neural cell adhesion molecules. / Wei, Ming Hui; Karavanova, Irina; Ivanov, Sergey V.; Popescu, Nicolae C.; Keck, Catherine L.; Pack, Svetlana; Eisen, Jonathan A; Lerman, Michael I.

In: Human Genetics, Vol. 103, No. 3, 1998, p. 355-364.

Research output: Contribution to journalArticle

Wei, Ming Hui ; Karavanova, Irina ; Ivanov, Sergey V. ; Popescu, Nicolae C. ; Keck, Catherine L. ; Pack, Svetlana ; Eisen, Jonathan A ; Lerman, Michael I. / In silico-initiated cloning and molecular characterization of a novel human member of the L1 gene family of neural cell adhesion molecules. In: Human Genetics. 1998 ; Vol. 103, No. 3. pp. 355-364.
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