In silico cloning of mouse Muc5b gene and upregulation of its expression in mouse asthma model

Yin Chen, Yu Hua Zhao, Reen Wu

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Using a BLAST-searching approach, we identified a mouse expressed sequence tag (EST) clone (AA038672) showing great similarity to the 3′ end of the human MUC5B gene. The clone was named "3pmmuc5b-1" after complete nucleotide sequencing (Genbank Accession, AF369933). A subsequent search of the mouse genome database with the 3pmmuc5b-1 sequence identified two overlapping genomic clones (AC020817 and AC020794) that contained the sequence of both 3pmmuc5b-1 and the mouse Muc5ac gene. Like their human homologs, the genomic order of the mouse Muc genes is 5′-Muc5ac-Muc5b-3′. These results suggest that the newly identified EST clone, 3pmmuc5b-1, is part of the 3′ portion of the mouse Muc5b gene. In situ hybridization demonstrated that this putative mouse Muc5b message was expressed in a restricted manner in the sublingual gland region of the tongue and the submucosal gland region of the mouse trachea in a normal animal. However, the gene expression was greatly enhanced in airway surface epithelium and the submucosal gland region in ovalbumin-induced asthmatic mice. These results were consistent with previous studies of human airway tissues. We therefore conclude that this newly cloned mouse Muc5b gene could be used as a marker for studying aberrant mucin gene expression in mouse models of various airway diseases.

Original languageEnglish (US)
Pages (from-to)1059-1066
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume164
Issue number6
StatePublished - Sep 15 2001

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Keywords

  • Airway epithelium
  • Asthma
  • Digital cloning
  • Gene expression
  • Mouse model
  • Mucin gene

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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