Abstract
Background: The temporomandibular joint (TMJ) is extremely important for activities like eating and talking, which can become painful and difficult for patients with TMJ dysfunction. Tissue engineering is a potential alternative to current surgical interventions through replacement of diseased or injured tissue with a functional construct. Since research with TMJ disc cells began relatively recently, optimal culturing conditions must be determined. Methods: Metabolic additives, L-glutamine, L-alanyl-L-glutamine, sodium pyruvate, and insulin, were examined for their effects on TMJ disc cells in monolayer. Effects of L-proline were examined in three-dimensional (3-D) culture at concentrations of 0, 25 and 100 mg/l. Results: The combination of L-glutamine, sodium pyruvate, and insulin improved cell proliferation rates without affecting collagen production or gene expression. No differences were observed in mechanical properties of the engineered constructs; however, collagen and glycosaminoglycan quantities normalized to cell number decreased at the highest concentration of L-proline. Conclusion: This work identified supplements for 2-D monolayer expansion. Other supplements or culture conditions still need to be investigated for 3-D tissue production. This work improves upon porcine TMJ disc cell culturing conditions, taking us closer to being able to engineer the TMJ disc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 246-257 |
| Number of pages | 12 |
| Journal | Cells Tissues Organs |
| Volume | 185 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2007 |
| Externally published | Yes |
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Keywords
- Temporomandibular joint disc
- Tissue culture
- Tissue engineering
ASJC Scopus subject areas
- Anatomy
Cite this
Improving culture conditions for temporomandibular joint disc tissue engineering. / Johns, D. E.; Athanasiou, K. A.
In: Cells Tissues Organs, Vol. 185, No. 4, 2007, p. 246-257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improving culture conditions for temporomandibular joint disc tissue engineering
AU - Johns, D. E.
AU - Athanasiou, K. A.
PY - 2007
Y1 - 2007
N2 - Background: The temporomandibular joint (TMJ) is extremely important for activities like eating and talking, which can become painful and difficult for patients with TMJ dysfunction. Tissue engineering is a potential alternative to current surgical interventions through replacement of diseased or injured tissue with a functional construct. Since research with TMJ disc cells began relatively recently, optimal culturing conditions must be determined. Methods: Metabolic additives, L-glutamine, L-alanyl-L-glutamine, sodium pyruvate, and insulin, were examined for their effects on TMJ disc cells in monolayer. Effects of L-proline were examined in three-dimensional (3-D) culture at concentrations of 0, 25 and 100 mg/l. Results: The combination of L-glutamine, sodium pyruvate, and insulin improved cell proliferation rates without affecting collagen production or gene expression. No differences were observed in mechanical properties of the engineered constructs; however, collagen and glycosaminoglycan quantities normalized to cell number decreased at the highest concentration of L-proline. Conclusion: This work identified supplements for 2-D monolayer expansion. Other supplements or culture conditions still need to be investigated for 3-D tissue production. This work improves upon porcine TMJ disc cell culturing conditions, taking us closer to being able to engineer the TMJ disc.
AB - Background: The temporomandibular joint (TMJ) is extremely important for activities like eating and talking, which can become painful and difficult for patients with TMJ dysfunction. Tissue engineering is a potential alternative to current surgical interventions through replacement of diseased or injured tissue with a functional construct. Since research with TMJ disc cells began relatively recently, optimal culturing conditions must be determined. Methods: Metabolic additives, L-glutamine, L-alanyl-L-glutamine, sodium pyruvate, and insulin, were examined for their effects on TMJ disc cells in monolayer. Effects of L-proline were examined in three-dimensional (3-D) culture at concentrations of 0, 25 and 100 mg/l. Results: The combination of L-glutamine, sodium pyruvate, and insulin improved cell proliferation rates without affecting collagen production or gene expression. No differences were observed in mechanical properties of the engineered constructs; however, collagen and glycosaminoglycan quantities normalized to cell number decreased at the highest concentration of L-proline. Conclusion: This work identified supplements for 2-D monolayer expansion. Other supplements or culture conditions still need to be investigated for 3-D tissue production. This work improves upon porcine TMJ disc cell culturing conditions, taking us closer to being able to engineer the TMJ disc.
KW - Temporomandibular joint disc
KW - Tissue culture
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=34250817362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250817362&partnerID=8YFLogxK
U2 - 10.1159/000102173
DO - 10.1159/000102173
M3 - Article
C2 - 17587799
AN - SCOPUS:34250817362
VL - 185
SP - 246
EP - 257
JO - Cells Tissues Organs
JF - Cells Tissues Organs
SN - 1422-6405
IS - 4
ER -