Improvements in diabetic microangiopathy after successful simultaneous pancreas-kidney transplantation

A computer-assisted intravital microscopy study on the conjunctival microcirculation

Anthony T W Cheung, Richard V Perez, Peter C Y Chen

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A computer-assisted intravital microscopy technology has been developed to noninvasively and objectively study diabetic microangiopathy in the conjunctival microcirculation of type-1 diabetics. Quantitative characterization of the conjunctival microcirculation was performed on 12 patients pre- and 18 months postsimultaneous pancreas-kidney transplantation (SPK). Healthy nondiabetic volunteers (n=12), solitary kidney (K) transplanted type-1 diabetics (n=5), and nontransplanted type-1 diabetics (n=12) served as controls. Pre-SPK diabetics showed abnormal-sized venules (diameter=66±7 μm) and reduced presence of arterioles (arteriole length/area=18±6 μm-1) compared with nondiabetic controls (53±4 μm; 31±8 μm-1; P<0.05). The computed vascular perfusion capacity of the conjunctival microvasculature was diminished in the same patients (pre-SPK diabetics=49±9%; nondiabetic healthy controls=71±6%; P<0.05). Significant improvement in microangiopathy was observed in all post-SPK diabetics (diameter=58±6 μm; arteriole length/area=26±9 μm-1; vascular perfusion=63±8%; P<0.05) 18 months post-SPK. Blood flow velocities in the conjunctival microcirculation in the same post-SPK patients showed noticeable but not significant improvements (nondiabetic controls=2.94±0.57 mm/sec; pre-SPK= 1.23±0.49 mm/sec; post-SPK=1.65±0.42 mm/sec). The solitary kidney transplant controls (post-K) showed no significant improvements in diabetic microangiopathy, confirming the unique role of the pancreas in SPK. In general, significant improvements (P<0.05) in diabetic microangiopathy were observed in all 12 diabetics 18 months post-SPK but not in the controls.

Original languageEnglish (US)
Pages (from-to)927-932
Number of pages6
JournalTransplantation
Volume68
Issue number7
DOIs
StatePublished - Oct 15 1999

Fingerprint

Diabetic Angiopathies
Pancreas Transplantation
Arterioles
Microcirculation
Kidney Transplantation
Blood Vessels
Perfusion
Kidney
Venules
Blood Flow Velocity
Microvessels
Pancreas
Healthy Volunteers
Technology
Transplants
Intravital Microscopy

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

@article{6688c95bd4884d34b208c81b915895f9,
title = "Improvements in diabetic microangiopathy after successful simultaneous pancreas-kidney transplantation: A computer-assisted intravital microscopy study on the conjunctival microcirculation",
abstract = "A computer-assisted intravital microscopy technology has been developed to noninvasively and objectively study diabetic microangiopathy in the conjunctival microcirculation of type-1 diabetics. Quantitative characterization of the conjunctival microcirculation was performed on 12 patients pre- and 18 months postsimultaneous pancreas-kidney transplantation (SPK). Healthy nondiabetic volunteers (n=12), solitary kidney (K) transplanted type-1 diabetics (n=5), and nontransplanted type-1 diabetics (n=12) served as controls. Pre-SPK diabetics showed abnormal-sized venules (diameter=66±7 μm) and reduced presence of arterioles (arteriole length/area=18±6 μm-1) compared with nondiabetic controls (53±4 μm; 31±8 μm-1; P<0.05). The computed vascular perfusion capacity of the conjunctival microvasculature was diminished in the same patients (pre-SPK diabetics=49±9{\%}; nondiabetic healthy controls=71±6{\%}; P<0.05). Significant improvement in microangiopathy was observed in all post-SPK diabetics (diameter=58±6 μm; arteriole length/area=26±9 μm-1; vascular perfusion=63±8{\%}; P<0.05) 18 months post-SPK. Blood flow velocities in the conjunctival microcirculation in the same post-SPK patients showed noticeable but not significant improvements (nondiabetic controls=2.94±0.57 mm/sec; pre-SPK= 1.23±0.49 mm/sec; post-SPK=1.65±0.42 mm/sec). The solitary kidney transplant controls (post-K) showed no significant improvements in diabetic microangiopathy, confirming the unique role of the pancreas in SPK. In general, significant improvements (P<0.05) in diabetic microangiopathy were observed in all 12 diabetics 18 months post-SPK but not in the controls.",
author = "Cheung, {Anthony T W} and Perez, {Richard V} and Chen, {Peter C Y}",
year = "1999",
month = "10",
day = "15",
doi = "10.1097/00007890-199910150-00005",
language = "English (US)",
volume = "68",
pages = "927--932",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Improvements in diabetic microangiopathy after successful simultaneous pancreas-kidney transplantation

T2 - A computer-assisted intravital microscopy study on the conjunctival microcirculation

AU - Cheung, Anthony T W

AU - Perez, Richard V

AU - Chen, Peter C Y

PY - 1999/10/15

Y1 - 1999/10/15

N2 - A computer-assisted intravital microscopy technology has been developed to noninvasively and objectively study diabetic microangiopathy in the conjunctival microcirculation of type-1 diabetics. Quantitative characterization of the conjunctival microcirculation was performed on 12 patients pre- and 18 months postsimultaneous pancreas-kidney transplantation (SPK). Healthy nondiabetic volunteers (n=12), solitary kidney (K) transplanted type-1 diabetics (n=5), and nontransplanted type-1 diabetics (n=12) served as controls. Pre-SPK diabetics showed abnormal-sized venules (diameter=66±7 μm) and reduced presence of arterioles (arteriole length/area=18±6 μm-1) compared with nondiabetic controls (53±4 μm; 31±8 μm-1; P<0.05). The computed vascular perfusion capacity of the conjunctival microvasculature was diminished in the same patients (pre-SPK diabetics=49±9%; nondiabetic healthy controls=71±6%; P<0.05). Significant improvement in microangiopathy was observed in all post-SPK diabetics (diameter=58±6 μm; arteriole length/area=26±9 μm-1; vascular perfusion=63±8%; P<0.05) 18 months post-SPK. Blood flow velocities in the conjunctival microcirculation in the same post-SPK patients showed noticeable but not significant improvements (nondiabetic controls=2.94±0.57 mm/sec; pre-SPK= 1.23±0.49 mm/sec; post-SPK=1.65±0.42 mm/sec). The solitary kidney transplant controls (post-K) showed no significant improvements in diabetic microangiopathy, confirming the unique role of the pancreas in SPK. In general, significant improvements (P<0.05) in diabetic microangiopathy were observed in all 12 diabetics 18 months post-SPK but not in the controls.

AB - A computer-assisted intravital microscopy technology has been developed to noninvasively and objectively study diabetic microangiopathy in the conjunctival microcirculation of type-1 diabetics. Quantitative characterization of the conjunctival microcirculation was performed on 12 patients pre- and 18 months postsimultaneous pancreas-kidney transplantation (SPK). Healthy nondiabetic volunteers (n=12), solitary kidney (K) transplanted type-1 diabetics (n=5), and nontransplanted type-1 diabetics (n=12) served as controls. Pre-SPK diabetics showed abnormal-sized venules (diameter=66±7 μm) and reduced presence of arterioles (arteriole length/area=18±6 μm-1) compared with nondiabetic controls (53±4 μm; 31±8 μm-1; P<0.05). The computed vascular perfusion capacity of the conjunctival microvasculature was diminished in the same patients (pre-SPK diabetics=49±9%; nondiabetic healthy controls=71±6%; P<0.05). Significant improvement in microangiopathy was observed in all post-SPK diabetics (diameter=58±6 μm; arteriole length/area=26±9 μm-1; vascular perfusion=63±8%; P<0.05) 18 months post-SPK. Blood flow velocities in the conjunctival microcirculation in the same post-SPK patients showed noticeable but not significant improvements (nondiabetic controls=2.94±0.57 mm/sec; pre-SPK= 1.23±0.49 mm/sec; post-SPK=1.65±0.42 mm/sec). The solitary kidney transplant controls (post-K) showed no significant improvements in diabetic microangiopathy, confirming the unique role of the pancreas in SPK. In general, significant improvements (P<0.05) in diabetic microangiopathy were observed in all 12 diabetics 18 months post-SPK but not in the controls.

UR - http://www.scopus.com/inward/record.url?scp=0033570285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033570285&partnerID=8YFLogxK

U2 - 10.1097/00007890-199910150-00005

DO - 10.1097/00007890-199910150-00005

M3 - Article

VL - 68

SP - 927

EP - 932

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 7

ER -