Improvement of recipient survival after small size graft liver transplantation in rats with preischemic manipulation or administering antisense against nuclear factor-κB

Jiang Ming Qian, Hao Zhang, Xiao Feng Wu, Guo Qiang Li, Xiao Ping Chen, Jian Wu

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The survival rate of small size graft liver transplantation (SSGLT) in rats is inversely related to graft volume. The present study aims to evaluate the protective effects of preischemic manipulation (PIM) and oligodeoxynucleotide (ODN) antisense against NF-κB on graft failure and animal survival. The protective effects of PIM and NF-κB ODN antisense were investigated in a rat SSGLT model. The graft function and survival of recipient animals over 3 weeks were monitored, and in situ staining for apoptotic cells in the graft tissue was examined. Both PIM and NF-κB antisense treatment significantly improved the survival of small graft-transplanted rats compared with the SSGLT group, lowered serum levels of alanine and aspartate aminotransferases, as well as tumor necrosis factor-α (TNF-α) levels, and minimized apoptotic cell counts in the liver sections. Moreover, the enhanced activation of NF-κB in the SSGLT group was diminished in both PIM and NF-κB antisense-treated groups. The findings suggest that enhanced NF-κB activation and TNF-α production may be involved in the ischemia/reperfusion-associated small size graft injury, and that PIM and antisense against NK-κB are effective in the attenuation of the small size graft injury, and improve the recipient animal survival.

Original languageEnglish (US)
Pages (from-to)784-789
Number of pages6
JournalTransplant International
Volume20
Issue number9
DOIs
StatePublished - Sep 2007

Keywords

  • Living donor liver transplantation
  • Nuclear factor-κB
  • Oligodeoxynucleotide antisense
  • Preischemic manipulation
  • Small size liver graft transplantation

ASJC Scopus subject areas

  • Transplantation

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