TY - JOUR
T1 - Improved survival of orthotopic liver allograft in swine by addition of trophic factors to University of Wisconsin solution
AU - Ambiru, Satoshi
AU - Uryuhara, Kenji
AU - Talpe, Stéphanie
AU - Dehoux, Jean Paul
AU - Jacobbi, Louise
AU - Murphy, Christopher J
AU - McAnulty, Jonathan F.
AU - Gianello, Pierre
PY - 2004/1/27
Y1 - 2004/1/27
N2 - Serum-free preservation media such as University of Wisconsin (UW) may cause tissue damage through trophic factor (TF) deprivation. This study evaluated whether the addition of TFs to UW solution improves liver graft quality after extended cold preservation time in pigs. UW solution was supplemented with epidermal growth factor, insulin-like growth factor-1, nerve growth factor-β, bactenecin, and substance P to create TF-supplemented (TFS) UW. Orthotopic liver transplantation was performed after 18 hr of static cold storage at 4°C in UW (n=7) or TFS-UW (n=7) solution. Recipients of grafts preserved with TFS-UW demonstrated significantly better 5-day survival (57%) than those preserved with UW alone (14%) (P<0.05). Adenosine triphosphate content in grafts preserved in TFS-UW was significantly higher than in grafts preserved in UW (17.4±5.0 vs. 4.8±1.2 nmol/mg protein, respectively) (P<0.05). This study showed that the addition of TFs to UW solution allowed a significant extension of cold ischemic time in pigs.
AB - Serum-free preservation media such as University of Wisconsin (UW) may cause tissue damage through trophic factor (TF) deprivation. This study evaluated whether the addition of TFs to UW solution improves liver graft quality after extended cold preservation time in pigs. UW solution was supplemented with epidermal growth factor, insulin-like growth factor-1, nerve growth factor-β, bactenecin, and substance P to create TF-supplemented (TFS) UW. Orthotopic liver transplantation was performed after 18 hr of static cold storage at 4°C in UW (n=7) or TFS-UW (n=7) solution. Recipients of grafts preserved with TFS-UW demonstrated significantly better 5-day survival (57%) than those preserved with UW alone (14%) (P<0.05). Adenosine triphosphate content in grafts preserved in TFS-UW was significantly higher than in grafts preserved in UW (17.4±5.0 vs. 4.8±1.2 nmol/mg protein, respectively) (P<0.05). This study showed that the addition of TFs to UW solution allowed a significant extension of cold ischemic time in pigs.
UR - http://www.scopus.com/inward/record.url?scp=0742304546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0742304546&partnerID=8YFLogxK
U2 - 10.1097/01.TP.0000100468.94126.AF
DO - 10.1097/01.TP.0000100468.94126.AF
M3 - Article
C2 - 14742997
AN - SCOPUS:0742304546
VL - 77
SP - 302
EP - 304
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 2
ER -