Improved separation and MS analysis of oligosaccharides by capillary electrophoresis

Hyun Joo An, Andreas H. Franz, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The separation of oligosaccharides using a combination of MALDI-mass spectrometry (MS) and capillary electrophoresis (CE) was discussed. The oligosaccharides were derivatized with benzylamine by reductive amination in the presence of NaCNBH 3. The benzylamine derivatized oligosaccharides were analyzed by CE/UV and off-line CE/MALDI-MS. The benzylamine labeled oligosaccharides were effectively detected at 230 and 248 nm. It was observed that the quaternization of benzylamine labled products enabled not only CE migration of neutral oligosaccharides but also separation of structural isomers of oligosaccharides.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages407-408
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

Fingerprint Dive into the research topics of 'Improved separation and MS analysis of oligosaccharides by capillary electrophoresis'. Together they form a unique fingerprint.

  • Cite this

    An, H. J., Franz, A. H., & Lebrilla, C. B. (2002). Improved separation and MS analysis of oligosaccharides by capillary electrophoresis. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 407-408)