Improved separation and MS analysis of oligosaccharides by capillary electrophoresis

Hyun Joo An; Andreas H. Franz; Carlito B Lebrilla

Improved separation and MS analysis of oligosaccharides by capillary electrophoresis

Hyun Joo An, Andreas H. Franz, Carlito B Lebrilla

Biochemistry and Molecular Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The separation of oligosaccharides using a combination of MALDI-mass spectrometry (MS) and capillary electrophoresis (CE) was discussed. The oligosaccharides were derivatized with benzylamine by reductive amination in the presence of NaCNBH ₃. The benzylamine derivatized oligosaccharides were analyzed by CE/UV and off-line CE/MALDI-MS. The benzylamine labeled oligosaccharides were effectively detected at 230 and 248 nm. It was observed that the quaternization of benzylamine labled products enabled not only CE migration of neutral oligosaccharides but also separation of structural isomers of oligosaccharides.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	407-408
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

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@inproceedings{e3cceab40c8f4272a8f1011ce6bb6acd,

title = "Improved separation and MS analysis of oligosaccharides by capillary electrophoresis",

abstract = "The separation of oligosaccharides using a combination of MALDI-mass spectrometry (MS) and capillary electrophoresis (CE) was discussed. The oligosaccharides were derivatized with benzylamine by reductive amination in the presence of NaCNBH 3. The benzylamine derivatized oligosaccharides were analyzed by CE/UV and off-line CE/MALDI-MS. The benzylamine labeled oligosaccharides were effectively detected at 230 and 248 nm. It was observed that the quaternization of benzylamine labled products enabled not only CE migration of neutral oligosaccharides but also separation of structural isomers of oligosaccharides.",

author = "An, {Hyun Joo} and Franz, {Andreas H.} and Lebrilla, {Carlito B}",

year = "2002",

language = "English (US)",

pages = "407--408",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Improved separation and MS analysis of oligosaccharides by capillary electrophoresis

AU - An, Hyun Joo

AU - Franz, Andreas H.

AU - Lebrilla, Carlito B

PY - 2002

Y1 - 2002

N2 - The separation of oligosaccharides using a combination of MALDI-mass spectrometry (MS) and capillary electrophoresis (CE) was discussed. The oligosaccharides were derivatized with benzylamine by reductive amination in the presence of NaCNBH 3. The benzylamine derivatized oligosaccharides were analyzed by CE/UV and off-line CE/MALDI-MS. The benzylamine labeled oligosaccharides were effectively detected at 230 and 248 nm. It was observed that the quaternization of benzylamine labled products enabled not only CE migration of neutral oligosaccharides but also separation of structural isomers of oligosaccharides.

AB - The separation of oligosaccharides using a combination of MALDI-mass spectrometry (MS) and capillary electrophoresis (CE) was discussed. The oligosaccharides were derivatized with benzylamine by reductive amination in the presence of NaCNBH 3. The benzylamine derivatized oligosaccharides were analyzed by CE/UV and off-line CE/MALDI-MS. The benzylamine labeled oligosaccharides were effectively detected at 230 and 248 nm. It was observed that the quaternization of benzylamine labled products enabled not only CE migration of neutral oligosaccharides but also separation of structural isomers of oligosaccharides.

UR - http://www.scopus.com/inward/record.url?scp=2442674498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442674498&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442674498

SP - 407

EP - 408

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -