Improved Radiolabeled Substrates for Soluble Epoxide Hydrolase

B. Borhan, T. Mebrahtu, S. Nazarian, M. J. Kurth, B. D. Hammock

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85 Scopus citations

Abstract

Two rapid assays for the soluble epoxide hydrolase (sEH) are described. First, a sensitive radiometric assay based on thin-layer chromatography of [14C]-cis-9,10-epoxystearic acid and its corresponding diol ([14C]-9,10-dihydroxystearic acid) is described. The cis fatty acid oxide exhibits higher specific activity of hydration with sEH from mouse, rat, human, and potato compared to trans-stilbene oxide (TSO). The Km and Vmax obtained for [14C]-cis-9,10-epoxystearic acid with mouse sEH are 11.0 μM and 3460 nmol/min/mg protein, respectively. [14C]-cis-9,10-Epoxystearic acid might more closely mimic the structures of natural substrates for sEH. Second, [2-3H]-trans-1,3-diphenylpropene oxide ([3H]-tDPPO) and [2-3H]-cis-1,3-diphenylpropene oxide ([3H]-cDPPO) were synthesized and rapid radiometric assays for epoxide hydrolases (EHs) were developed by differential partitioning of the epoxide into iso-octane and its corresponding diol into aqueous phase containing methanol. It was shown that sEHs from mouse, rat, human, and potato rapidly hydrolyze [3H]-tDPPO and in comparison to TSO have 20-, 49-, 28-, and 7-fold higher rates, respectively. Mouse sEH hydrates [3H]-tDPPO at 26,200 nmol/min/mg protein, and a Km of 2.80 μM is observed.

Original languageEnglish (US)
Pages (from-to)188-200
Number of pages13
JournalAnalytical Biochemistry
Volume231
Issue number1
DOIs
StatePublished - Oct 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

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    Borhan, B., Mebrahtu, T., Nazarian, S., Kurth, M. J., & Hammock, B. D. (1995). Improved Radiolabeled Substrates for Soluble Epoxide Hydrolase. Analytical Biochemistry, 231(1), 188-200. https://doi.org/10.1006/abio.1995.1520