Improved pulsed-field gel electrophoresis procedure for the analysis of Flavobacterium columnare isolates previously affected by DNA degradation

Esteban Soto Martinez, Michael Mauel, Mark Lawrence

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Flavobacterium columnare is a fresh water bacterium that causes columnaris diseases in over 36 fish species. Intra-species typing of F. columnare can be performed by pulsed-field gel electrophoresis (PFGE). However, this method is hampered by the degradation of chromosomal DNA in about 10% of strains. In the current study, DNA degradation problems caused by extracellular DNases were overcome by fixation of cells with formaldehyde prior to isolation. The results substantiate that after problems due to DNases are overcome, PFGE analysis is a reproducible highly discriminating epidemiological method for studying F. columnare isolates regardless of fish host.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalVeterinary Microbiology
Volume128
Issue number1-2
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

Keywords

  • DNA degradation
  • Flavobacterium columnare
  • Formaldehyde fixation
  • PFGE

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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