Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

Musleh M. Muthana, Jingyao Qu, Mengyang Xue, Timofey Klyuchnik, Alex Siu, Yanhong Li, Lei Zhang, Hai Yu, Lei Li, Peng G. Wang, Xi Chen

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD<sup>+</sup>)-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules. This journal is

Original languageEnglish (US)
Pages (from-to)4595-4598
Number of pages4
JournalChemical Communications
Volume51
Issue number22
DOIs
StatePublished - Mar 18 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

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    Muthana, M. M., Qu, J., Xue, M., Klyuchnik, T., Siu, A., Li, Y., Zhang, L., Yu, H., Li, L., Wang, P. G., & Chen, X. (2015). Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides. Chemical Communications, 51(22), 4595-4598. https://doi.org/10.1039/c4cc10306h