Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

Musleh M. Muthana, Jingyao Qu, Mengyang Xue, Timofey Klyuchnik, Alex Siu, Yanhong Li, Lei Zhang, Hai Yu, Lei Li, Peng G. Wang, Xi Chen

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD<sup>+</sup>)-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules. This journal is

Original languageEnglish (US)
Pages (from-to)4595-4598
Number of pages4
JournalChemical Communications
Issue number22
StatePublished - Mar 18 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys


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