Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin

Caitlin Campbell, Dmitry Grapov, Oliver Fiehn, Carol J. Chandler, Dustin J. Burnett, Elaine C. Souza, Gretchen A. Casazza, Mary B. Gustafson, Nancy L. Keim, John W. Newman, Gary R. Hunter, Jose R. Fernandez, W. Timothy Garvey, Mary Ellen Harper, Charles L. Hoppel, John K. Meissen, Kohei Take, Sean H. Adams

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Novel plasma metabolite patterns reflective of improved metabolic health (insulin sensitivity, fitness, reduced body weight) were identified before and after a 14-17 wk weight loss and exercise intervention in sedentary, obese insulin-resistant women. To control for potential confounding effects of diet- or microbiome-derived molecules on the systemic metabolome, sampling was during a tightly-controlled feeding test week paradigm. Pairwise and multivariate analysis revealed intervention- and insulin-sensitivity associated: (1) Changes in plasma xeno-metabolites ("non-self" metabolites of dietary or gut microbial origin) following an oral glucose tolerance test (e.g. higher post-OGTT propane-1,2,3-tricarboxylate [tricarballylic acid]) or in the overnight-fasted state (e.g., lower γ-tocopherol); (2) Increased indices of saturated very long chain fatty acid elongation capacity; (3) Increased post-OGTT α-ketoglutaric acid (α-KG), fasting α-KG inversely correlated with Matsuda index, and altered patterns of malate, pyruvate and glutamine hypothesized to stem from improved mitochondrial efficiency and more robust oxidation of glucose. The results support a working model in which improved metabolic health modifies host metabolism in parallel with altering systemic exposure to xeno-metabolites. This highlights that interpretations regarding the origins of peripheral blood or urinary "signatures" of insulin resistance and metabolic health must consider the potentially important contribution of gut-derived metabolites toward the host's metabolome.

Original languageEnglish (US)
Article numbere84260
JournalPLoS One
Volume9
Issue number1
DOIs
StatePublished - Jan 8 2014

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Glucose Tolerance Test
Metabolites
Metabolism
Insulin Resistance
Metabolome
insulin resistance
digestive system
Health
metabolites
metabolism
Insulin
metabolome
Ketoglutaric Acids
Tocopherols
Microbiota
Glutamine
Pyruvic Acid
Weight Loss
Fasting
very long chain fatty acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Campbell, C., Grapov, D., Fiehn, O., Chandler, C. J., Burnett, D. J., Souza, E. C., ... Adams, S. H. (2014). Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin. PLoS One, 9(1), [e84260]. https://doi.org/10.1371/journal.pone.0084260

Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin. / Campbell, Caitlin; Grapov, Dmitry; Fiehn, Oliver; Chandler, Carol J.; Burnett, Dustin J.; Souza, Elaine C.; Casazza, Gretchen A.; Gustafson, Mary B.; Keim, Nancy L.; Newman, John W.; Hunter, Gary R.; Fernandez, Jose R.; Garvey, W. Timothy; Harper, Mary Ellen; Hoppel, Charles L.; Meissen, John K.; Take, Kohei; Adams, Sean H.

In: PLoS One, Vol. 9, No. 1, e84260, 08.01.2014.

Research output: Contribution to journalArticle

Campbell, C, Grapov, D, Fiehn, O, Chandler, CJ, Burnett, DJ, Souza, EC, Casazza, GA, Gustafson, MB, Keim, NL, Newman, JW, Hunter, GR, Fernandez, JR, Garvey, WT, Harper, ME, Hoppel, CL, Meissen, JK, Take, K & Adams, SH 2014, 'Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin', PLoS One, vol. 9, no. 1, e84260. https://doi.org/10.1371/journal.pone.0084260
Campbell, Caitlin ; Grapov, Dmitry ; Fiehn, Oliver ; Chandler, Carol J. ; Burnett, Dustin J. ; Souza, Elaine C. ; Casazza, Gretchen A. ; Gustafson, Mary B. ; Keim, Nancy L. ; Newman, John W. ; Hunter, Gary R. ; Fernandez, Jose R. ; Garvey, W. Timothy ; Harper, Mary Ellen ; Hoppel, Charles L. ; Meissen, John K. ; Take, Kohei ; Adams, Sean H. / Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin. In: PLoS One. 2014 ; Vol. 9, No. 1.
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