Abstract
Juvenile hormone esterase (JHE), a member of the carboxylesterase family (EC 3.1.1.1), metabolizes JH that is found in juvenile insects. A highly conserved amphipathic alpha helix is found on the surface of known JHEs. This helix is implicated in receptor-mediated binding and endocytosis of JHE by the pericardial cells resulting in the clearance of JHE activity from the hemolymph. In this study, Lys-204 and Arg-208 of the amphipathic alpha helix of the JHE of Manduca sexta (MsJHE) were mutated to histidine residues generating MsJHE-HH. Pharmacokinetic studies following the injection of MsJHE-HH into the hemocoel of larval M. sexta, Heliothis virescens, and Agrotis ipsilon indicated that MsJHE-HH and wild type MsJHE are cleared at similar rates. The infectivity (lethal concentration and lethal time) of a recombinant baculovirus, AcMsJHE-HH, expressing MsJHE-HH was not significantly different than that of a recombinant baculovirus, AcMsJHE, expressing MsJHE in first instars of H. virescens and A. ipsilon. However, the mass of AcMsJHE-HH-infected larvae was 40-50% lower than that of larvae infected with AcMsJHE, and 70-90% lower than that of wild type AcMNPV-infected larvae.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 354-361 |
| Number of pages | 8 |
| Journal | Biological Control |
| Volume | 58 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2011 |
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Keywords
- Baculovirus
- JH
- JHE
- Juvenile hormone esterase
ASJC Scopus subject areas
- Agronomy and Crop Science
- Insect Science
Cite this
Improved insecticidal efficacy of a recombinant baculovirus expressing mutated JH esterase from Manduca sexta. / El-Sheikh, El Sayed A; Kamita, Shizuo G.; Vu, Kiem; Hammock, Bruce D.
In: Biological Control, Vol. 58, No. 3, 09.2011, p. 354-361.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved insecticidal efficacy of a recombinant baculovirus expressing mutated JH esterase from Manduca sexta
AU - El-Sheikh, El Sayed A
AU - Kamita, Shizuo G.
AU - Vu, Kiem
AU - Hammock, Bruce D.
PY - 2011/9
Y1 - 2011/9
N2 - Juvenile hormone esterase (JHE), a member of the carboxylesterase family (EC 3.1.1.1), metabolizes JH that is found in juvenile insects. A highly conserved amphipathic alpha helix is found on the surface of known JHEs. This helix is implicated in receptor-mediated binding and endocytosis of JHE by the pericardial cells resulting in the clearance of JHE activity from the hemolymph. In this study, Lys-204 and Arg-208 of the amphipathic alpha helix of the JHE of Manduca sexta (MsJHE) were mutated to histidine residues generating MsJHE-HH. Pharmacokinetic studies following the injection of MsJHE-HH into the hemocoel of larval M. sexta, Heliothis virescens, and Agrotis ipsilon indicated that MsJHE-HH and wild type MsJHE are cleared at similar rates. The infectivity (lethal concentration and lethal time) of a recombinant baculovirus, AcMsJHE-HH, expressing MsJHE-HH was not significantly different than that of a recombinant baculovirus, AcMsJHE, expressing MsJHE in first instars of H. virescens and A. ipsilon. However, the mass of AcMsJHE-HH-infected larvae was 40-50% lower than that of larvae infected with AcMsJHE, and 70-90% lower than that of wild type AcMNPV-infected larvae.
AB - Juvenile hormone esterase (JHE), a member of the carboxylesterase family (EC 3.1.1.1), metabolizes JH that is found in juvenile insects. A highly conserved amphipathic alpha helix is found on the surface of known JHEs. This helix is implicated in receptor-mediated binding and endocytosis of JHE by the pericardial cells resulting in the clearance of JHE activity from the hemolymph. In this study, Lys-204 and Arg-208 of the amphipathic alpha helix of the JHE of Manduca sexta (MsJHE) were mutated to histidine residues generating MsJHE-HH. Pharmacokinetic studies following the injection of MsJHE-HH into the hemocoel of larval M. sexta, Heliothis virescens, and Agrotis ipsilon indicated that MsJHE-HH and wild type MsJHE are cleared at similar rates. The infectivity (lethal concentration and lethal time) of a recombinant baculovirus, AcMsJHE-HH, expressing MsJHE-HH was not significantly different than that of a recombinant baculovirus, AcMsJHE, expressing MsJHE in first instars of H. virescens and A. ipsilon. However, the mass of AcMsJHE-HH-infected larvae was 40-50% lower than that of larvae infected with AcMsJHE, and 70-90% lower than that of wild type AcMNPV-infected larvae.
KW - Baculovirus
KW - JH
KW - JHE
KW - Juvenile hormone esterase
UR - http://www.scopus.com/inward/record.url?scp=79960348841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960348841&partnerID=8YFLogxK
U2 - 10.1016/j.biocontrol.2011.06.002
DO - 10.1016/j.biocontrol.2011.06.002
M3 - Article
AN - SCOPUS:79960348841
VL - 58
SP - 354
EP - 361
JO - Biological Control
JF - Biological Control
SN - 1049-9644
IS - 3
ER -