Improved insecticidal efficacy of a recombinant baculovirus expressing mutated JH esterase from Manduca sexta

El Sayed A El-Sheikh, Shizuo G. Kamita, Kiem Vu, Bruce D. Hammock

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Juvenile hormone esterase (JHE), a member of the carboxylesterase family (EC 3.1.1.1), metabolizes JH that is found in juvenile insects. A highly conserved amphipathic alpha helix is found on the surface of known JHEs. This helix is implicated in receptor-mediated binding and endocytosis of JHE by the pericardial cells resulting in the clearance of JHE activity from the hemolymph. In this study, Lys-204 and Arg-208 of the amphipathic alpha helix of the JHE of Manduca sexta (MsJHE) were mutated to histidine residues generating MsJHE-HH. Pharmacokinetic studies following the injection of MsJHE-HH into the hemocoel of larval M. sexta, Heliothis virescens, and Agrotis ipsilon indicated that MsJHE-HH and wild type MsJHE are cleared at similar rates. The infectivity (lethal concentration and lethal time) of a recombinant baculovirus, AcMsJHE-HH, expressing MsJHE-HH was not significantly different than that of a recombinant baculovirus, AcMsJHE, expressing MsJHE in first instars of H. virescens and A. ipsilon. However, the mass of AcMsJHE-HH-infected larvae was 40-50% lower than that of larvae infected with AcMsJHE, and 70-90% lower than that of wild type AcMNPV-infected larvae.

Original languageEnglish (US)
Pages (from-to)354-361
Number of pages8
JournalBiological Control
Volume58
Issue number3
DOIs
StatePublished - Sep 2011

Fingerprint

juvenile hormone esterase
Manduca sexta
Baculoviridae
Agrotis ipsilon
carboxylesterase
Heliothis virescens
larvae
Autographa californica multiple nucleopolyhedrovirus
hemocoel
endocytosis
histidine
pharmacokinetics
hemolymph
pathogenicity
instars
injection
insects
receptors
cells

Keywords

  • Baculovirus
  • JH
  • JHE
  • Juvenile hormone esterase

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Improved insecticidal efficacy of a recombinant baculovirus expressing mutated JH esterase from Manduca sexta. / El-Sheikh, El Sayed A; Kamita, Shizuo G.; Vu, Kiem; Hammock, Bruce D.

In: Biological Control, Vol. 58, No. 3, 09.2011, p. 354-361.

Research output: Contribution to journalArticle

El-Sheikh, El Sayed A ; Kamita, Shizuo G. ; Vu, Kiem ; Hammock, Bruce D. / Improved insecticidal efficacy of a recombinant baculovirus expressing mutated JH esterase from Manduca sexta. In: Biological Control. 2011 ; Vol. 58, No. 3. pp. 354-361.
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