Improved collection and developmental competence of immature macaque oocytes

C. A. VandeVoort, S. P. Leibo, Alice F Tarantal

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Methods previously described to aspirate immature oocytes from ovaries of macaques result in approximately half the oocytes being stripped of cumulus cells. Here, we describe modifications of the needle aspiration assembly that yield much higher percentages of cumulus-intact oocytes when used with an ultrasound-guided method for oocyte recovery in monkeys. Sealing of the needle assembly appears to stabilize vacuum pressure at the needle tip and prevents air from entering the tubing. Reduction of the vacuum pressure from -100 to -20 kPa resulted in a significant decrease of denuded oocytes from over 50% to fewer than 10%. This was accompanied by a significant increase in the percentage of oocytes that developed into blastocysts after in vitro fertilization. Reduction of the aspiration pressure below -20 kPa significantly reduced the total number of oocytes recovered. We concluded that these modifications represent the best compromise to collect the largest number of cumulus-intact oocyte complexes from macaques.

Original languageEnglish (US)
Pages (from-to)699-707
Number of pages9
JournalTheriogenology
Volume59
Issue number3-4
DOIs
StatePublished - Feb 2003

Keywords

  • Assisted reproduction
  • Cumulus-oocyte complex
  • In vitro maturation
  • Macaca mulatta
  • Ultrasound

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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