Improved capillary electrophoretic separation and mass spectrometric detection of oligosaccharides

Hyun Joo An, Andreas H. Franz, Carlito B Lebrilla

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have developed a CE method for the separation of structural isomers of oligosaccharides labeled with N-quaternized benzylamine. Oligosaccharides with reducing ends were derivatized with benzylamine by reductive amination followed by quaternization to yield a fixed cation label. The benzylamine-derivatized oligosaccharides were analyzed by CE-UV in ammonium acetate buffer and off-line matrix-assisted laser desorption ionization (MALDI) MS. The method was applied to a 1 nmol sample of a model oligosaccharide (LNDFH 1). From this sample a 38 fmol diluted standard was detected. The quaternization of benzylamine-labeled products significantly improved CE separation of neutral oligosaccharides along with several structural isomers. Two hexasaccharide isomers (LNDFH I and LNDFH II) were baseline resolved using an ammonium acetate buffer. This method was also applied successfully to the profiling of oligosaccharides released from the glycoprotein RNase B. The release of 6 pmol of glycans followed by workup showed the detection of all components, with one component corresponding to 100 fmol. Micropreparative collection of CE enabled successful off-line CE-MALDI-MS without additional sample clean up. This report provides a simple and rapid method to separate and analyze oligosaccharides.

Original languageEnglish (US)
Pages (from-to)121-129
Number of pages9
JournalJournal of Chromatography A
Volume1004
Issue number1-2
DOIs
StatePublished - Jul 4 2003

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Oligosaccharides
Isomers
Ionization
Desorption
Buffers
Lasers
Amination
Polysaccharides
Cations
Labels
Glycoproteins
benzylamine

Keywords

  • Derivatization, electrophoresis
  • Glycoproteins
  • Oligosaccharides
  • Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Improved capillary electrophoretic separation and mass spectrometric detection of oligosaccharides. / An, Hyun Joo; Franz, Andreas H.; Lebrilla, Carlito B.

In: Journal of Chromatography A, Vol. 1004, No. 1-2, 04.07.2003, p. 121-129.

Research output: Contribution to journalArticle

An, Hyun Joo ; Franz, Andreas H. ; Lebrilla, Carlito B. / Improved capillary electrophoretic separation and mass spectrometric detection of oligosaccharides. In: Journal of Chromatography A. 2003 ; Vol. 1004, No. 1-2. pp. 121-129.
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