Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules

Diane M. Sheridan, Roslyn Rivkah Isseroff, Richard Nuccitelli

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response to an electric migratory signal. Cultured human keratinocytes were plated on different matrices: types I and TV collagen, fibronectin, laminin, and tissue culture plastic. The effect of an applied direct current (DC) electric held on directional migration was monitored by time-lapse video microscopy over a 2-h period. Directionality was quantitated by calculating the cosine of the angle of migration in relation to anodal-cathodal orientation. Migration toward the negative pole was observed on all matrices as compared with controls (no applied held), which displayed random migration, No significant increase in directional response occurred when the held strength was increased from 100 mV/mm (physiologic levels) to 400 mV/mm. The degree of directionality and the average net cell translocation, however, varied significantly with the substrate. The greatest cathodal migration in response to a DC electric field was observed with keratinocytes plated on types I and IV collagens and plastic. The directional migratory response was least on a laminin substrate, whereas cells on fibronectin demonstrated a response that was intermediate between those of collagen and laminin. These results suggest that physiologic ionic currents in concert with the underlying matrix may influence the rate of reepithelialization of skin wounds.

Original languageEnglish (US)
Pages (from-to)642-646
Number of pages5
JournalJournal of Investigative Dermatology
Volume106
Issue number4
StatePublished - 1996

Fingerprint

Keratinocytes
Extracellular Matrix
Laminin
Electric fields
Molecules
Collagen Type I
Fibronectins
Plastics
Skin
Substrates
Collagen
Video Microscopy
Tissue culture
Collagen Type IV
Wounds and Injuries
Electric currents
Wound Healing
Poles
Microscopic examination

Keywords

  • Collagen
  • Galvanotaxis
  • Motility
  • Wound healing

ASJC Scopus subject areas

  • Dermatology

Cite this

Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules. / Sheridan, Diane M.; Isseroff, Roslyn Rivkah; Nuccitelli, Richard.

In: Journal of Investigative Dermatology, Vol. 106, No. 4, 1996, p. 642-646.

Research output: Contribution to journalArticle

@article{4b527b9ae71d45de853d17c4e0e2c6a4,
title = "Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules",
abstract = "Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response to an electric migratory signal. Cultured human keratinocytes were plated on different matrices: types I and TV collagen, fibronectin, laminin, and tissue culture plastic. The effect of an applied direct current (DC) electric held on directional migration was monitored by time-lapse video microscopy over a 2-h period. Directionality was quantitated by calculating the cosine of the angle of migration in relation to anodal-cathodal orientation. Migration toward the negative pole was observed on all matrices as compared with controls (no applied held), which displayed random migration, No significant increase in directional response occurred when the held strength was increased from 100 mV/mm (physiologic levels) to 400 mV/mm. The degree of directionality and the average net cell translocation, however, varied significantly with the substrate. The greatest cathodal migration in response to a DC electric field was observed with keratinocytes plated on types I and IV collagens and plastic. The directional migratory response was least on a laminin substrate, whereas cells on fibronectin demonstrated a response that was intermediate between those of collagen and laminin. These results suggest that physiologic ionic currents in concert with the underlying matrix may influence the rate of reepithelialization of skin wounds.",
keywords = "Collagen, Galvanotaxis, Motility, Wound healing",
author = "Sheridan, {Diane M.} and Isseroff, {Roslyn Rivkah} and Richard Nuccitelli",
year = "1996",
language = "English (US)",
volume = "106",
pages = "642--646",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules

AU - Sheridan, Diane M.

AU - Isseroff, Roslyn Rivkah

AU - Nuccitelli, Richard

PY - 1996

Y1 - 1996

N2 - Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response to an electric migratory signal. Cultured human keratinocytes were plated on different matrices: types I and TV collagen, fibronectin, laminin, and tissue culture plastic. The effect of an applied direct current (DC) electric held on directional migration was monitored by time-lapse video microscopy over a 2-h period. Directionality was quantitated by calculating the cosine of the angle of migration in relation to anodal-cathodal orientation. Migration toward the negative pole was observed on all matrices as compared with controls (no applied held), which displayed random migration, No significant increase in directional response occurred when the held strength was increased from 100 mV/mm (physiologic levels) to 400 mV/mm. The degree of directionality and the average net cell translocation, however, varied significantly with the substrate. The greatest cathodal migration in response to a DC electric field was observed with keratinocytes plated on types I and IV collagens and plastic. The directional migratory response was least on a laminin substrate, whereas cells on fibronectin demonstrated a response that was intermediate between those of collagen and laminin. These results suggest that physiologic ionic currents in concert with the underlying matrix may influence the rate of reepithelialization of skin wounds.

AB - Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response to an electric migratory signal. Cultured human keratinocytes were plated on different matrices: types I and TV collagen, fibronectin, laminin, and tissue culture plastic. The effect of an applied direct current (DC) electric held on directional migration was monitored by time-lapse video microscopy over a 2-h period. Directionality was quantitated by calculating the cosine of the angle of migration in relation to anodal-cathodal orientation. Migration toward the negative pole was observed on all matrices as compared with controls (no applied held), which displayed random migration, No significant increase in directional response occurred when the held strength was increased from 100 mV/mm (physiologic levels) to 400 mV/mm. The degree of directionality and the average net cell translocation, however, varied significantly with the substrate. The greatest cathodal migration in response to a DC electric field was observed with keratinocytes plated on types I and IV collagens and plastic. The directional migratory response was least on a laminin substrate, whereas cells on fibronectin demonstrated a response that was intermediate between those of collagen and laminin. These results suggest that physiologic ionic currents in concert with the underlying matrix may influence the rate of reepithelialization of skin wounds.

KW - Collagen

KW - Galvanotaxis

KW - Motility

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=9244228499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9244228499&partnerID=8YFLogxK

M3 - Article

VL - 106

SP - 642

EP - 646

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 4

ER -