Importance of membrane selection in the development of immunochromatographic assays for low-molecular weight compounds

Ji Ye Lee, Young Ah Kim, Mi Yeon Kim, Yong Tae Lee, Bruce D. Hammock, Hye Sung Lee

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

This study was performed to demonstrate the importance of selecting an appropriate membrane when developing immunochromatographic assays (ICAs) for the sensitive detection of low-molecular weight compounds. Based on our findings, we propose a theoretical basis for selecting such a membrane. When eluting the sample solution for the competitive ICA using colloidal gold label for low-molecular analytes, the degree of binding inhibition is proportional to the collision frequency between the antibody-colloidal gold (Ab-CG) and analyte before Ab-CG binding to the capture antigen and a higher concentration of pesticides around the Ab-CG leads to a greater degree of inhibition. Therefore, we propose that the relative migration speed of the analyte and Ab-CG on the test strip is critically important for selecting a membrane in the development of sensitive competitive ICAs. We developed a novel method to estimate such a relative migration speed. We demonstrated the applicability of this proposal by using it to select an appropriate membrane for the development of an ICA of the pesticide diazinon.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalAnalytica Chimica Acta
Volume757
DOIs
StatePublished - Dec 13 2012

Keywords

  • Diazinon
  • Immunochromatographic assay
  • Low-molecular weight compounds
  • Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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