Abstract
The murine dorsum dermal excisional wound model has been widely utilized with or without splint application. However, variations in experimental methods create challenges for direct comparison of results provided in the literature and for design of new wound healing studies. Here, we investigated the effects of wound location and size, number of wounds, type of adhesive used for splint fixation on wound healing using splinted or unsplinted dorsum excisional full thickness wound models. One or two 6- or 8-mm full thickness wounds were made with or without splinting in genetically diabetic but heterozygous mice (Dock7<sup>m</sup>-+-/-+-Lepr<sup>db</sup>). Two different adhesives: tissue adhesive and an over the counter cyanoacrylate adhesive (OTCA) "Krazy glue" were used to fix splints. Wound contraction, wound closure, and histopathological parameters including reepithelialization, collagen deposition and inflammation were compared between groups. No significant effect of wound number (1 vs. 2), side (left vs. right and cranial vs. caudal) or size on wound healing was observed. The OTCA group had a significantly higher splint success compared to the tissue adhesive group that resulted in significantly higher reepithelialization and collagen deposition in the OTCA group. Understanding the outcomes and effects of the variables will help investigators choose appropriate experimental conditions for the study purpose and interpret data.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 251-261 |
| Number of pages | 11 |
| Journal | Wound Repair and Regeneration |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1 2015 |
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ASJC Scopus subject areas
- Dermatology
- Surgery
Cite this
Importance of defining experimental conditions in a mouse excisional wound model. / Park, Shin Ae; Covert, Jill; Teixeira, Leandro; Motta, Monica J.; Deremer, Sara L.; Abbott, Nicholas L.; Dubielzig, Richard; Schurr, Michael; Isseroff, Roslyn Rivkah; McAnulty, Jonathan F.; Murphy, Christopher J.
In: Wound Repair and Regeneration, Vol. 23, No. 2, 01.03.2015, p. 251-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Importance of defining experimental conditions in a mouse excisional wound model
AU - Park, Shin Ae
AU - Covert, Jill
AU - Teixeira, Leandro
AU - Motta, Monica J.
AU - Deremer, Sara L.
AU - Abbott, Nicholas L.
AU - Dubielzig, Richard
AU - Schurr, Michael
AU - Isseroff, Roslyn Rivkah
AU - McAnulty, Jonathan F.
AU - Murphy, Christopher J
PY - 2015/3/1
Y1 - 2015/3/1
N2 - The murine dorsum dermal excisional wound model has been widely utilized with or without splint application. However, variations in experimental methods create challenges for direct comparison of results provided in the literature and for design of new wound healing studies. Here, we investigated the effects of wound location and size, number of wounds, type of adhesive used for splint fixation on wound healing using splinted or unsplinted dorsum excisional full thickness wound models. One or two 6- or 8-mm full thickness wounds were made with or without splinting in genetically diabetic but heterozygous mice (Dock7m-+-/-+-Leprdb). Two different adhesives: tissue adhesive and an over the counter cyanoacrylate adhesive (OTCA) "Krazy glue" were used to fix splints. Wound contraction, wound closure, and histopathological parameters including reepithelialization, collagen deposition and inflammation were compared between groups. No significant effect of wound number (1 vs. 2), side (left vs. right and cranial vs. caudal) or size on wound healing was observed. The OTCA group had a significantly higher splint success compared to the tissue adhesive group that resulted in significantly higher reepithelialization and collagen deposition in the OTCA group. Understanding the outcomes and effects of the variables will help investigators choose appropriate experimental conditions for the study purpose and interpret data.
AB - The murine dorsum dermal excisional wound model has been widely utilized with or without splint application. However, variations in experimental methods create challenges for direct comparison of results provided in the literature and for design of new wound healing studies. Here, we investigated the effects of wound location and size, number of wounds, type of adhesive used for splint fixation on wound healing using splinted or unsplinted dorsum excisional full thickness wound models. One or two 6- or 8-mm full thickness wounds were made with or without splinting in genetically diabetic but heterozygous mice (Dock7m-+-/-+-Leprdb). Two different adhesives: tissue adhesive and an over the counter cyanoacrylate adhesive (OTCA) "Krazy glue" were used to fix splints. Wound contraction, wound closure, and histopathological parameters including reepithelialization, collagen deposition and inflammation were compared between groups. No significant effect of wound number (1 vs. 2), side (left vs. right and cranial vs. caudal) or size on wound healing was observed. The OTCA group had a significantly higher splint success compared to the tissue adhesive group that resulted in significantly higher reepithelialization and collagen deposition in the OTCA group. Understanding the outcomes and effects of the variables will help investigators choose appropriate experimental conditions for the study purpose and interpret data.
UR - http://www.scopus.com/inward/record.url?scp=84931062053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84931062053&partnerID=8YFLogxK
U2 - 10.1111/wrr.12272
DO - 10.1111/wrr.12272
M3 - Article
C2 - 25703258
AN - SCOPUS:84931062053
VL - 23
SP - 251
EP - 261
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
SN - 1067-1927
IS - 2
ER -