Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae

Tze Hsien Toh, Gerald Kayingo, Marthinus J. Van Der Merwe, Stephanus G. Kilian, John E. Hallsworth, Stefan Hohmann, Bernard A. Prior

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The deletion of the gene encoding the glycerol facilitator Fps1p was associated with an altered plasma membrane lipid composition in Saccharomyces cerevisiae. The S. cerevisiae fps1Δ strain respectively contained 18 and 26% less ergosterol than the wild-type strain, at the whole-cell level and at the plasma membrane level. Other mutants with deficiencies in glycerol metabolism were studied to investigate any possible link between membrane ergosterol content and intracellular glycerol accumulation. In these mutants a modification in intracellular glycerol concentration, or in intra- to extracellular glycerol ratio was accompanied by a reduction in plasma membrane ergosterol content. However, there was no direct correlation between ergosterol content and intracellular glycerol concentration. Lipid composition influences the membrane permeability for solutes during adaptation of yeast cells to osmotic stress. In this study, ergosterol supplementation was shown to partially suppress the hypo-osmotic sensitivity phenotype of the fps1Δ strain, leading to more efficient glycerol efflux, and improved survival. The erg-1 disruption mutant, which is unable to synthesise ergosterol, survived and recovered from the hypo-osmotic shock more successfully when the concentration of exogenously supplied ergosterol was increased. The results obtained suggest that a higher ergosterol content facilitates the flux of glycerol across the plasma membrane of S. cerevisiae cells.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalFEMS Yeast Research
Volume1
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Ergosterol
Glycerol
Saccharomyces cerevisiae
Membranes
Cell Membrane
Osmotic Pressure
Gene Deletion
Membrane Lipids
Permeability
Yeasts
Phenotype
Lipids

Keywords

  • Ergosterol
  • Fps1 glycerol channel
  • Glycerol efflux
  • Hypo-osmotic stress

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae. / Toh, Tze Hsien; Kayingo, Gerald; Van Der Merwe, Marthinus J.; Kilian, Stephanus G.; Hallsworth, John E.; Hohmann, Stefan; Prior, Bernard A.

In: FEMS Yeast Research, Vol. 1, No. 3, 2001, p. 205-211.

Research output: Contribution to journalArticle

Toh, Tze Hsien ; Kayingo, Gerald ; Van Der Merwe, Marthinus J. ; Kilian, Stephanus G. ; Hallsworth, John E. ; Hohmann, Stefan ; Prior, Bernard A. / Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae. In: FEMS Yeast Research. 2001 ; Vol. 1, No. 3. pp. 205-211.
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