Impaired proteostasis in senescent vascular endothelial cells: a perspective on estrogen and oxidative stress in the aging vasculature

Hyun Tae V. Hwang, Yun Lin, Michelle N. Rebuffatti, Darlene T. Tran, Lily Lee, Aldrin V Gomes, Chin-Shang Li, Anne A Knowlton

Research output: Contribution to journalArticle

Abstract

The heat shock response is an important cytoprotective mechanism for protein homeostasis and is an essential protective response to cellular stress and injury. Studies on changes in the heat shock response with aging have been mixed with regard to whether it is inhibited, and this, at least in part, reflects different tissues and different models. Cellular senescence is a key feature in aging, but work on the heat shock response in cultured senescent (SEN) cells has largely been limited to fibroblasts. Given the prevalence of oxidative injury in the aging cardiovascular system, we investigated whether SEN primary human coronary artery endothelial cells have a diminished heat shock response and impaired proteostasis. In addition, we tested whether this downregulation of heat shock response can be mitigated by 17β-estradiol (E2), which has a critical cardioprotective role in women, as we have previously reported that E2 improves the heat shock response in endothelial cells (Hamilton KL, Mbai FN, Gupta S, Knowlton AA. Arterioscler Thromb Vasc Biol 24: 1628-1633, 2004). We found that SEN endothelial cells, despite their unexpectedly increased proteasome activity, had a diminished heat shock response and had more protein aggregation than early passage cells. SEN cells had increased oxidative stress, which promoted protein aggregation. E2 treatment did not decrease protein aggregation or improve the heat shock response in either early passage or SEN cells. In summary, cellular senescence in adult human endothelial cells is accompanied by increased oxidative stress and a blunting of proteostasis, and E2 did not mitigate these changes. NEW & NOTEWORTHY Senescent human endothelial cells have a diminished heat shock response and increased protein aggregates. Senescent human endothelial cells have increased basal oxidative stress, which increases protein aggregates. Physiological level of 17β-estradiol did not improve proteostasis in endothelial cells.

Original languageEnglish (US)
Pages (from-to)H421-H429
JournalAmerican journal of physiology. Heart and circulatory physiology
Volume316
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Heat-Shock Response
Estrogens
Oxidative Stress
Endothelial Cells
Cell Aging
Estradiol
Proteins
Wounds and Injuries
Proteasome Endopeptidase Complex
Cardiovascular System
Cultured Cells
Coronary Vessels
Homeostasis
Down-Regulation
Fibroblasts

Keywords

  • endothelial cell
  • estrogen
  • heat shock response
  • proteostasis
  • senescence

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Impaired proteostasis in senescent vascular endothelial cells : a perspective on estrogen and oxidative stress in the aging vasculature. / Hwang, Hyun Tae V.; Lin, Yun; Rebuffatti, Michelle N.; Tran, Darlene T.; Lee, Lily; Gomes, Aldrin V; Li, Chin-Shang; Knowlton, Anne A.

In: American journal of physiology. Heart and circulatory physiology, Vol. 316, No. 2, 01.02.2019, p. H421-H429.

Research output: Contribution to journalArticle

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