Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and sarcoplasmic reticulum Ca2+ loading in quiescent junctophilin 1 knock-out myotubes

Hongli Li, Xudong Ding, Jose R. Lopez, Hiroshi Takeshima, Jianjie Ma, Paul D. Allen, Jose M. Eltit

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


In the absence of store depletion, plasmalemmal Ca2+ permeability in resting muscle is very low, and its contribution in the maintenance of Ca2+ homeostasis at rest has not been studied in detail. Junctophilin 1 knock-out myotubes (JP1 KO) have a severe reduction in store-operated Ca2+ entry, presumably caused by physical alteration of the sarcoplasmic reticulum (SR) and T-tubule junction, leading to disruption of the SR signal sent by Stim1 to activate Orai1. Using JP1 KO myotubes as a model, we assessed the contribution of the Orai1-mediated Ca2+ entry pathway on overall Ca2+ homeostasis at rest with no store depletion. JP1 KO myotubes have decreased Ca2+ entry, [Ca2+] rest, and intracellular Ca2+ content compared with WT myotubes and unlike WT myotubes, are refractory to BTP2, a Ca2+ entry blocker. JP1 KO myotubes show down-regulation of Orai1 and Stim1 proteins, suggesting that this pathway may be important in the control of resting Ca 2+ homeostasis. WT myotubes stably transduced with Orai1(E190Q) had similar alterations in their resting Ca2+ homeostasis as JP1 KO myotubes and were also unresponsive to BTP2. JP1 KO cells show decreased expression of TRPC1 and -3 but overexpress TRPC4 and -6; on the other hand, the TRPC expression profile in Orai1(E190Q) myotubes was comparable with WT. These data suggest that an important fraction of resting plasmalemmal Ca2+ permeability is mediated by the Orai1 pathway, which contributes to the control of [Ca2+]rest and resting Ca2+ stores and that this pathway is defective in JP1 KO myotubes.

Original languageEnglish (US)
Pages (from-to)39171-39179
Number of pages9
JournalJournal of Biological Chemistry
Issue number50
StatePublished - Dec 10 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology


Dive into the research topics of 'Impaired Orai1-mediated resting Ca<sup>2+</sup> entry reduces the cytosolic [Ca<sup>2+</sup>] and sarcoplasmic reticulum Ca<sup>2+</sup> loading in quiescent junctophilin 1 knock-out myotubes'. Together they form a unique fingerprint.

Cite this