Impact of polydonor mixed lymphocyte culture media on quantity and quality of myeloid metaphases

Nancy Taylor, Jeanna L Welborn, Jerry P. Lewis

Research output: Contribution to journalArticle


A novel mitotic stimulator of myeloid hematopoietic cells was prospectively evaluated to detect enhanced quantity and quality of metaphases from bone marrow (BM) cultures for cytogenetic analysis. This polydonor mixed lymphocyte conditioned (PMLC) media decreased the culture failure rate, improved detection of chromosomal aberrations, and reduced technologists' analysis time. The conditioned media is recommended as a highly effective culture system for normal and neoplastic cells of myeloid lineage.

Original languageEnglish (US)
Pages (from-to)46-49
Number of pages4
JournalCancer Genetics and Cytogenetics
Issue number1
StatePublished - Jul 1 1992


ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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