Impact of polydonor mixed lymphocyte culture media on quantity and quality of myeloid metaphases

Nancy Taylor, Jeanna L Welborn, Jerry P. Lewis

Research output: Contribution to journalArticle

Abstract

A novel mitotic stimulator of myeloid hematopoietic cells was prospectively evaluated to detect enhanced quantity and quality of metaphases from bone marrow (BM) cultures for cytogenetic analysis. This polydonor mixed lymphocyte conditioned (PMLC) media decreased the culture failure rate, improved detection of chromosomal aberrations, and reduced technologists' analysis time. The conditioned media is recommended as a highly effective culture system for normal and neoplastic cells of myeloid lineage.

Original languageEnglish (US)
Pages (from-to)46-49
Number of pages4
JournalCancer Genetics and Cytogenetics
Volume61
Issue number1
DOIs
StatePublished - Jul 1 1992

Fingerprint

Metaphase
Conditioned Culture Medium
Culture Media
Lymphocytes
Cytogenetic Analysis
Cell Lineage
Myeloid Cells
Chromosome Aberrations
Bone Marrow

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Impact of polydonor mixed lymphocyte culture media on quantity and quality of myeloid metaphases. / Taylor, Nancy; Welborn, Jeanna L; Lewis, Jerry P.

In: Cancer Genetics and Cytogenetics, Vol. 61, No. 1, 01.07.1992, p. 46-49.

Research output: Contribution to journalArticle

@article{fb3313edfc204e15bead71f023ff3d4d,
title = "Impact of polydonor mixed lymphocyte culture media on quantity and quality of myeloid metaphases",
abstract = "A novel mitotic stimulator of myeloid hematopoietic cells was prospectively evaluated to detect enhanced quantity and quality of metaphases from bone marrow (BM) cultures for cytogenetic analysis. This polydonor mixed lymphocyte conditioned (PMLC) media decreased the culture failure rate, improved detection of chromosomal aberrations, and reduced technologists' analysis time. The conditioned media is recommended as a highly effective culture system for normal and neoplastic cells of myeloid lineage.",
author = "Nancy Taylor and Welborn, {Jeanna L} and Lewis, {Jerry P.}",
year = "1992",
month = "7",
day = "1",
doi = "10.1016/0165-4608(92)90369-J",
language = "English (US)",
volume = "61",
pages = "46--49",
journal = "Cancer Genetics and Cytogenetics",
issn = "0165-4608",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Impact of polydonor mixed lymphocyte culture media on quantity and quality of myeloid metaphases

AU - Taylor, Nancy

AU - Welborn, Jeanna L

AU - Lewis, Jerry P.

PY - 1992/7/1

Y1 - 1992/7/1

N2 - A novel mitotic stimulator of myeloid hematopoietic cells was prospectively evaluated to detect enhanced quantity and quality of metaphases from bone marrow (BM) cultures for cytogenetic analysis. This polydonor mixed lymphocyte conditioned (PMLC) media decreased the culture failure rate, improved detection of chromosomal aberrations, and reduced technologists' analysis time. The conditioned media is recommended as a highly effective culture system for normal and neoplastic cells of myeloid lineage.

AB - A novel mitotic stimulator of myeloid hematopoietic cells was prospectively evaluated to detect enhanced quantity and quality of metaphases from bone marrow (BM) cultures for cytogenetic analysis. This polydonor mixed lymphocyte conditioned (PMLC) media decreased the culture failure rate, improved detection of chromosomal aberrations, and reduced technologists' analysis time. The conditioned media is recommended as a highly effective culture system for normal and neoplastic cells of myeloid lineage.

UR - http://www.scopus.com/inward/record.url?scp=0026716992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026716992&partnerID=8YFLogxK

U2 - 10.1016/0165-4608(92)90369-J

DO - 10.1016/0165-4608(92)90369-J

M3 - Article

VL - 61

SP - 46

EP - 49

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

SN - 0165-4608

IS - 1

ER -