Impact of genetics and environment on the metabolite composition of maize grain

Kirsten Skogerson, George G. Harrigan, Tracey L. Reynolds, Steven C. Halls, Martin Ruebelt, Alberto Iandolino, Anand Pandravada, Kevin C. Glenn, Oliver Fiehn

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

This study sought to assess genetic and environmental impacts on the metabolite composition of maize grain. Gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS) measured 119 Identified metabolites Including free amino acids, free fatty acids, sugars, organic acids, and other small molecules in a range of hybrids derived from 48 Inbred lines crossed against two different tester lines (from the C103 and lodent heterotic groups) and grown at three locations in Iowa. It was reasoned that expanded metabolite coverage would contribute to a comprehensive evaluation of the grain metabolome, its degree of variability, and, in principle, its relationship to other compositional and agronomic features. The metabolic profiling results established that the small molecule metabolite pool is highly dependent on genotypic variation and that levels of certain metabolite classes may have an inverse genotypic relationship to each other. Different metabolic phenotypes were clearly associated with the two distinct tester populations. Overall, grain from the C103 lines contained higher levels of free fatty acids and organic acids, whereas grain from the lodent lines were associated with higher levels of amino acids and carbohydrates. In addition, the fold-range of genotype mean values [composed of six samples each (two tester crosses per inbred x three field sites)] for identified metabolites ranged from 1.5- to 93-fold. Interestingly, some grain metabolites showed a non-normal distribution over the entire corn population, which could, at least In part, be attributed to large differences in metabolite values within specific inbred crosses relative to other Inbred sets. This study suggests a potential role for metabolic profiling in assisting the process of selecting elite germplasm in biotechnology development, or markerassisted breeding.

Original languageEnglish (US)
Pages (from-to)3600-3610
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number6
DOIs
StatePublished - Mar 24 2010

Keywords

  • Maize
  • Metaboiomics
  • Metabolic profiling
  • Natural variation
  • Zea mays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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    Skogerson, K., Harrigan, G. G., Reynolds, T. L., Halls, S. C., Ruebelt, M., Iandolino, A., Pandravada, A., Glenn, K. C., & Fiehn, O. (2010). Impact of genetics and environment on the metabolite composition of maize grain. Journal of Agricultural and Food Chemistry, 58(6), 3600-3610. https://doi.org/10.1021/jf903705y