Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines

James S. Felton, Mark G. Knize, L. Michelle Bennett, Michael A. Malfatti, Michael E. Colvin, Kristen S. Kulp

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Carcinogenic heterocyclic amines are produced from overcooked foods and are highly mutagenic in most short-term test systems. One of the most abundant of these amines, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), induces breast, colon and prostate tumors in rats. Human dietary epidemiology studies suggest a strong correlation between either meat consumption or well-done muscle meat consumption and cancers of the colon, breast, stomach, lung and esophagus. For over 20 years our laboratory has helped define the human exposure to these dietary carcinogens. In this report we describe how various environmental exposures may modulate the risk from exposure to heterocyclic amines, especially PhIP. To assess the impact of foods on PhIP metabolism in humans, we developed an LC/MS/MS method to analyze the four major PhIP urinary metabolites following the consumption of a single portion of grilled chicken. Adding broccoli to the volunteers' diet altered the kinetics of PhIP metabolism. At the cellular level we have found that PhIP itself stimulates a significant estrogenic response in MCF-7 cells, but even more interestingly, co-incubation of the cells with herbal teas appear to enhance the response. Numerous environmental chemicals found in food or the atmosphere can impact the exposure, metabolism, and cell proliferation response of heterocyclic amines.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalToxicology
Volume198
Issue number1-3
DOIs
StatePublished - May 20 2004
Externally publishedYes

Fingerprint

Environmental Exposure
Amines
Metabolism
Meats
Food
Meat
Enzyme kinetics
Epidemiology
Brassica
MCF-7 Cells
Cell proliferation
Nutrition
Metabolites
2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
Atmosphere
Carcinogens
Colonic Neoplasms
Esophagus
Muscle
Rats

Keywords

  • Carcinogenicity
  • Heterocyclic amines
  • Mutagenicity
  • PhIP

ASJC Scopus subject areas

  • Toxicology

Cite this

Felton, J. S., Knize, M. G., Bennett, L. M., Malfatti, M. A., Colvin, M. E., & Kulp, K. S. (2004). Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines. Toxicology, 198(1-3), 135-145. https://doi.org/10.1016/j.tox.2004.01.024

Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines. / Felton, James S.; Knize, Mark G.; Bennett, L. Michelle; Malfatti, Michael A.; Colvin, Michael E.; Kulp, Kristen S.

In: Toxicology, Vol. 198, No. 1-3, 20.05.2004, p. 135-145.

Research output: Contribution to journalArticle

Felton, JS, Knize, MG, Bennett, LM, Malfatti, MA, Colvin, ME & Kulp, KS 2004, 'Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines', Toxicology, vol. 198, no. 1-3, pp. 135-145. https://doi.org/10.1016/j.tox.2004.01.024
Felton JS, Knize MG, Bennett LM, Malfatti MA, Colvin ME, Kulp KS. Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines. Toxicology. 2004 May 20;198(1-3):135-145. https://doi.org/10.1016/j.tox.2004.01.024
Felton, James S. ; Knize, Mark G. ; Bennett, L. Michelle ; Malfatti, Michael A. ; Colvin, Michael E. ; Kulp, Kristen S. / Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines. In: Toxicology. 2004 ; Vol. 198, No. 1-3. pp. 135-145.
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