Impact of culture conditions on tissue remodeling in 3-D collagen gels

Angelie Agarwal, Steven George

Research output: Contribution to journalConference articlepeer-review


We compared gel contraction patterns in two different culture conditions: (1) human lung fibroblast cell line (CCD-18 lu) and transformed bronchial epithelial cells (16HBE14o-), and (2) normal human lung fibroblasts (NHLF) and small airway epithelial cells (SAEC). Data are expressed as percent of initial area. Gels without an epithelium had a higher degree of contraction than gels with an epithelium (CCD-18 = 16.9% vs. CCD-18 + 16HBE14o- = 29.9%, NHLF = 8.3% vs. NHLF + SAEC = 25.2%). Similar results were found for the rate constant of gel contraction (CCD-18 = 3.3 days vs. CCD-18 + 16HBE14o- = 4.2 days, NHLF = 1.9 days vs. NHLF + SAEC = 2.2 days). Results are consistent with epithelial inhibition of TGF-b production. (NSF BES-9875033).

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Issue numberSUPPL. 1
StatePublished - Dec 1 2000
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering


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