Impact of alpha-tocopherol deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses

Lauren Bookbinder, Carrie J. Finno, Anna M. Firshman, Scott A. Katzman, Erin Burns, Janel Peterson, Anna Dahlgren, Brittni Ming-Whitfield, Shelby Glessner, Amanda Borer-Matsui, Stephanie J. Valberg

Research output: Contribution to journalArticle

Abstract

Background: A subset of horses deficient in alpha-tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). Objectives: To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. Animals: Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases. Methods: Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. Results: Mitochondrial alterations more common than our reference range (<2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. Conclusions and Clinical Importance: Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP deficiency may be of long-term importance for mitochondrial viability.

Original languageEnglish (US)
Pages (from-to)2770-2779
Number of pages10
JournalJournal of Veterinary Internal Medicine
Volume33
Issue number6
DOIs
StatePublished - Nov 1 2019

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muscular diseases
alpha-Tocopherol
muscle fibers
histopathology
alpha-tocopherol
Horses
vitamin E
Vitamin E
Muscular Diseases
horses
Muscles
atrophy
Atrophy
muscles
Mitochondrial Myopathies
Quarter Horse
muscular atrophy
Muscular Atrophy
prospective studies
retrospective studies

Keywords

  • atrophy
  • mitochondria
  • myopathy
  • skeletal muscle
  • vitamin E

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Impact of alpha-tocopherol deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses. / Bookbinder, Lauren; Finno, Carrie J.; Firshman, Anna M.; Katzman, Scott A.; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J.

In: Journal of Veterinary Internal Medicine, Vol. 33, No. 6, 01.11.2019, p. 2770-2779.

Research output: Contribution to journalArticle

Bookbinder, L, Finno, CJ, Firshman, AM, Katzman, SA, Burns, E, Peterson, J, Dahlgren, A, Ming-Whitfield, B, Glessner, S, Borer-Matsui, A & Valberg, SJ 2019, 'Impact of alpha-tocopherol deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses', Journal of Veterinary Internal Medicine, vol. 33, no. 6, pp. 2770-2779. https://doi.org/10.1111/jvim.15643
Bookbinder, Lauren ; Finno, Carrie J. ; Firshman, Anna M. ; Katzman, Scott A. ; Burns, Erin ; Peterson, Janel ; Dahlgren, Anna ; Ming-Whitfield, Brittni ; Glessner, Shelby ; Borer-Matsui, Amanda ; Valberg, Stephanie J. / Impact of alpha-tocopherol deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses. In: Journal of Veterinary Internal Medicine. 2019 ; Vol. 33, No. 6. pp. 2770-2779.
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abstract = "Background: A subset of horses deficient in alpha-tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). Objectives: To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. Animals: Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases. Methods: Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. Results: Mitochondrial alterations more common than our reference range (<2.5{\%} affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0{\%}-10{\%} of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13{\%} ± 7{\%}; GM, 3{\%} ± 2{\%}) and anguloid atrophy compared to subclinical day 0 horses. Conclusions and Clinical Importance: Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP deficiency may be of long-term importance for mitochondrial viability.",
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T1 - Impact of alpha-tocopherol deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses

AU - Bookbinder, Lauren

AU - Finno, Carrie J.

AU - Firshman, Anna M.

AU - Katzman, Scott A.

AU - Burns, Erin

AU - Peterson, Janel

AU - Dahlgren, Anna

AU - Ming-Whitfield, Brittni

AU - Glessner, Shelby

AU - Borer-Matsui, Amanda

AU - Valberg, Stephanie J.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Background: A subset of horses deficient in alpha-tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). Objectives: To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. Animals: Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases. Methods: Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. Results: Mitochondrial alterations more common than our reference range (<2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. Conclusions and Clinical Importance: Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP deficiency may be of long-term importance for mitochondrial viability.

AB - Background: A subset of horses deficient in alpha-tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). Objectives: To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. Animals: Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases. Methods: Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. Results: Mitochondrial alterations more common than our reference range (<2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. Conclusions and Clinical Importance: Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP deficiency may be of long-term importance for mitochondrial viability.

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KW - mitochondria

KW - myopathy

KW - skeletal muscle

KW - vitamin E

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