Immunoproteomic identification of bovine pericardium xenoantigens

Leigh G. Griffiths, Leila H. Choe, Kenneth F. Reardon, Steven W. Dow, E. Christopher Orton

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bovine pericardium is an important biomaterial with current application in glutaraldehyde-fixed bioprosthetic heart valves and possible future application as an unfixed biological scaffold for tissue engineering. The importance of both humoral and cell-mediated rejection responses toward fixed and unfixed xenogeneic tissues has become increasingly apparent. However, the full scope and specific identities of bovine pericardium proteins that can elicit an immune response remain largely unknown. In this study, an immunoproteomic approach was used to survey bovine pericardium proteins for their ability to elicit a humoral immune response in rabbits. A two-stage protein extraction protocol was used to separate bovine pericardium proteins into water- and lipid-soluble fractions. Two-dimensional (2-D) gel electrophoresis was performed to separate the proteins from each fraction. Western blots were generated from 2-D gels of both bovine pericardium protein fractions. These blots were probed with serum from rabbits immunized with bovine pericardium and a secondary antibody was used to assess for IgG positivity. Western blots were compared to duplicate 2-D gels and proteins in matched spots were identified by tandem mass spectrometry. Thirty-one putative protein antigens were identified, eight of which are known to be antigenic from previous studies. All of the putative antigens demonstrated progressive staining intensity with increasing days of post-exposure serum. Identified antigenic proteins represented a variety of functional and structural protein types, and included both cellular and matrix proteins. The results of this study have implications for the use of bovine pericardium as a biomaterial in bioprostheses and tissue engineering applications, as well as xenotransplantation in general.

Original languageEnglish (US)
Pages (from-to)3514-3520
Number of pages7
JournalBiomaterials
Volume29
Issue number26
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Heterophile Antigens
Pericardium
Proteins
Gels
Biocompatible Materials
Tissue Engineering
Antigens
Tissue engineering
Biomaterials
Western Blotting
Rabbits
Bioprosthesis
Heterologous Transplantation
Heart Valves
Electrophoresis, Gel, Two-Dimensional
Glutaral
Scaffolds (biology)
Humoral Immunity
Tandem Mass Spectrometry
Electrophoresis

Keywords

  • Heart valve
  • Immunoproteomics
  • Scaffold
  • Tissue engineering
  • Xenoantigens

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Griffiths, L. G., Choe, L. H., Reardon, K. F., Dow, S. W., & Christopher Orton, E. (2008). Immunoproteomic identification of bovine pericardium xenoantigens. Biomaterials, 29(26), 3514-3520. https://doi.org/10.1016/j.biomaterials.2008.05.006

Immunoproteomic identification of bovine pericardium xenoantigens. / Griffiths, Leigh G.; Choe, Leila H.; Reardon, Kenneth F.; Dow, Steven W.; Christopher Orton, E.

In: Biomaterials, Vol. 29, No. 26, 09.2008, p. 3514-3520.

Research output: Contribution to journalArticle

Griffiths, LG, Choe, LH, Reardon, KF, Dow, SW & Christopher Orton, E 2008, 'Immunoproteomic identification of bovine pericardium xenoantigens', Biomaterials, vol. 29, no. 26, pp. 3514-3520. https://doi.org/10.1016/j.biomaterials.2008.05.006
Griffiths LG, Choe LH, Reardon KF, Dow SW, Christopher Orton E. Immunoproteomic identification of bovine pericardium xenoantigens. Biomaterials. 2008 Sep;29(26):3514-3520. https://doi.org/10.1016/j.biomaterials.2008.05.006
Griffiths, Leigh G. ; Choe, Leila H. ; Reardon, Kenneth F. ; Dow, Steven W. ; Christopher Orton, E. / Immunoproteomic identification of bovine pericardium xenoantigens. In: Biomaterials. 2008 ; Vol. 29, No. 26. pp. 3514-3520.
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