Immunophenotypical characterization of monocytes in canine distemper virus infection

Veronika M. Stein, Nicole M S Schreiner, Peter F Moore, Marc Vandevelde, Andreas Zurbriggen, Andrea Tipold

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Canine distemper virus (CDV) infection induces multifocal demyelination in the central nervous system (CNS). It is thought that the resident macrophages of the CNS, the microglia, as well as invading monocytes associated with the inflammatory reaction may play a central role in the demyelinating process. To evaluate changes in peripheral monocytes in CDV infection their immunophenotype was characterized by flow cytometry during the course of an experimental CDV infection in dogs. The highest number of CDV-infected monocytes was found in dogs developing demyelinating lesions. In CD18, CD45, CD44, and CD14 neither up- nor down-regulation was observed. Marked up-regulation occurred in a number of surface molecules including CD1c, B7-1 and B7-2, MHC I, and CD11b. Peak expression was found at 4-5 weeks post-infection (PI), regardless of clinical outcome. All these molecules play an important role in the host's immune response, notably antigen presentation and cell adhesion. These results demonstrate that CDV infection in vivo may enhance several macrophage functions. This could lead to more effective clearance of the virus but may also increase demyelination through a bystander effect in animals that accumulated significant amounts of CDV in the CNS.

Original languageEnglish (US)
Pages (from-to)237-246
Number of pages10
JournalVeterinary Microbiology
Volume131
Issue number3-4
DOIs
StatePublished - Oct 15 2008
Externally publishedYes

Fingerprint

Canine Distemper Virus
Canine distemper virus
Virus Diseases
monocytes
Monocytes
infection
central nervous system
Central Nervous System
Demyelinating Diseases
macrophages
Macrophages
Dogs
Bystander Effect
antigen presentation
dogs
Histocompatibility Antigens Class II
Antigen Presentation
neuroglia
Microglia
cell adhesion

Keywords

  • Canine distemper virus infection (CDV)
  • Canine monocytes
  • Immunophenotype
  • Pathogenesis of CDV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Stein, V. M., Schreiner, N. M. S., Moore, P. F., Vandevelde, M., Zurbriggen, A., & Tipold, A. (2008). Immunophenotypical characterization of monocytes in canine distemper virus infection. Veterinary Microbiology, 131(3-4), 237-246. https://doi.org/10.1016/j.vetmic.2008.03.009

Immunophenotypical characterization of monocytes in canine distemper virus infection. / Stein, Veronika M.; Schreiner, Nicole M S; Moore, Peter F; Vandevelde, Marc; Zurbriggen, Andreas; Tipold, Andrea.

In: Veterinary Microbiology, Vol. 131, No. 3-4, 15.10.2008, p. 237-246.

Research output: Contribution to journalArticle

Stein, VM, Schreiner, NMS, Moore, PF, Vandevelde, M, Zurbriggen, A & Tipold, A 2008, 'Immunophenotypical characterization of monocytes in canine distemper virus infection', Veterinary Microbiology, vol. 131, no. 3-4, pp. 237-246. https://doi.org/10.1016/j.vetmic.2008.03.009
Stein, Veronika M. ; Schreiner, Nicole M S ; Moore, Peter F ; Vandevelde, Marc ; Zurbriggen, Andreas ; Tipold, Andrea. / Immunophenotypical characterization of monocytes in canine distemper virus infection. In: Veterinary Microbiology. 2008 ; Vol. 131, No. 3-4. pp. 237-246.
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