Immunophenotypic and cytomorphologic subclassification of T-cell lymphoma in the boxer breed

David M. Lurie, Rowan J. Milner, Steven E. Suter, William Vernau

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The boxer breed is at high risk for developing lymphoma and, in contrast to the general canine population, is predisposed to the T-cell variant of the disease. The purpose of this study was to more accurately classify lymphoma in this breed. Clinical, cytomorphologic and immunophenotypic data were examined in 43 boxers with lymphoma. Twenty-five cases were collected prospectively and a further 18 cases were obtained retrospectively. Lymphomas were classified as multicentric (n = 29), mediastinal (n = 6) and intestinal (n = 8). Of the 40 immunophenotyped samples, 34 (85%) were T-cell, 5 (12.5%) were B-cell and 1 was a non-B-cell non-T-cell lymphoma. Immunophenotypic subtyping was done on prospectively collected T-cell lymphoma samples (n = 22) to differentiate CD4 (helper) from CD8 (cytotoxic) T-cell origin as well as to determine the T-cell receptor (TCR) expression (TCRαβ vs. TCRδγ). Phenotypic expression was CD4+ (n = 12), double negative (DN) (n = 6), double positive (DP) (n = 1) and CD8+ (n = 1), respectively, while two samples had no interpretable result. 20/22 samples were TCRαβ+ with only 1 sample being TCRδγ+ and 1 with no interpretable result. Cytomorphologic analysis was done on the same 22 samples using the World Health Organization (WHO) classification scheme. According to this scheme, 17/22 samples were classified as lymphoblastic, 2/22 as large cell peripheral T-cell lymphoma-not otherwise specified (PTCL-NOS), 2/22 as large granular lymphoma (LGL) high-grade and 1/22 as small lymphocytic. The results of this study indicate that lymphoma in the boxer breed is a disease comprised predominantly of TCRαβ+, CD4+ (helper) T-cells with lymphoblastic (high-grade) morphology.

Original languageEnglish (US)
Pages (from-to)102-110
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume125
Issue number1-2
DOIs
StatePublished - Sep 15 2008

Fingerprint

Boxer (dog breed)
T-Cell Lymphoma
T-Cell Antigen Receptor
lymphoma
Lymphoma
T-lymphocytes
breeds
T-Lymphocytes
receptors
Peripheral T-Cell Lymphoma
sampling
Helper-Inducer T-Lymphocytes
Non-Hodgkin's Lymphoma
Canidae
B-Lymphocytes
World Health Organization
cells
Population
B-lymphocytes

Keywords

  • Boxer
  • Cytomorphology
  • Immunophenotype
  • Lymphoma

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Immunophenotypic and cytomorphologic subclassification of T-cell lymphoma in the boxer breed. / Lurie, David M.; Milner, Rowan J.; Suter, Steven E.; Vernau, William.

In: Veterinary Immunology and Immunopathology, Vol. 125, No. 1-2, 15.09.2008, p. 102-110.

Research output: Contribution to journalArticle

Lurie, David M. ; Milner, Rowan J. ; Suter, Steven E. ; Vernau, William. / Immunophenotypic and cytomorphologic subclassification of T-cell lymphoma in the boxer breed. In: Veterinary Immunology and Immunopathology. 2008 ; Vol. 125, No. 1-2. pp. 102-110.
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