TY - JOUR
T1 - Immunophenotype and gene expression profile of mesenchymal stem cells derived from canine adipose tissue and bone marrow
AU - Screven, Rudell
AU - Kenyon, Elizabeth
AU - Myers, Michael J.
AU - Yancy, Haile F.
AU - Skasko, Mark
AU - Boxer, Lynne
AU - Bigley, Elmer C.
AU - Borjesson, Dori L
AU - Zhu, Min
PY - 2014/9/15
Y1 - 2014/9/15
N2 - Veterinary adult stem cell therapy is an emerging area of basic and clinical research. Like their human counterparts, veterinary mesenchymal stem cells (MSCs) offer many potential therapeutic benefits. The characterization of canine-derived MSCs, however, is poorly defined compared to human MSCs. Furthermore, little consensus exists regarding the expression of canine MSC cell surface markers. To address this issue, this study investigated characteristics of cultured canine MSCs derived from both adipose tissue and bone marrow. The canine MSCs were obtained from donors of various breeds and ages. A panel of cell surface markers for canine MSCs was selected based on current human and canine literature and the availability of canine-reactive antibodies. Using flow cytometry, canine MSCs were defined to be CD90+CD44+MHC I+CD14-CD29-CD34-MHC II-. Canine MSCs were further characterized using real-time RT-PCR as CD105+CD73+CD14+CD29+MHC II+CD45- at the mRNA level. Among these markers, canine MSCs differed from canine peripheral blood mononuclear cells (PBMCs) by the absence of CD45 expression at the mRNA level. A novel high-throughput canine-specific PCR array was developed and used to identify changes in the gene expression profiles of canine MSCs. Genes including PTPRC, TNF, β2M, TGFβ1, and PDGFRβ, were identified as unique to canine MSCs as compared to canine PBMCs. Our findings will facilitate characterization of canine MSCs for use in research and clinical trials. Moreover, the high-throughput PCR array is a novel tool for characterizing canine MSCs isolated from different tissues and potentially from different laboratories.
AB - Veterinary adult stem cell therapy is an emerging area of basic and clinical research. Like their human counterparts, veterinary mesenchymal stem cells (MSCs) offer many potential therapeutic benefits. The characterization of canine-derived MSCs, however, is poorly defined compared to human MSCs. Furthermore, little consensus exists regarding the expression of canine MSC cell surface markers. To address this issue, this study investigated characteristics of cultured canine MSCs derived from both adipose tissue and bone marrow. The canine MSCs were obtained from donors of various breeds and ages. A panel of cell surface markers for canine MSCs was selected based on current human and canine literature and the availability of canine-reactive antibodies. Using flow cytometry, canine MSCs were defined to be CD90+CD44+MHC I+CD14-CD29-CD34-MHC II-. Canine MSCs were further characterized using real-time RT-PCR as CD105+CD73+CD14+CD29+MHC II+CD45- at the mRNA level. Among these markers, canine MSCs differed from canine peripheral blood mononuclear cells (PBMCs) by the absence of CD45 expression at the mRNA level. A novel high-throughput canine-specific PCR array was developed and used to identify changes in the gene expression profiles of canine MSCs. Genes including PTPRC, TNF, β2M, TGFβ1, and PDGFRβ, were identified as unique to canine MSCs as compared to canine PBMCs. Our findings will facilitate characterization of canine MSCs for use in research and clinical trials. Moreover, the high-throughput PCR array is a novel tool for characterizing canine MSCs isolated from different tissues and potentially from different laboratories.
KW - Canine
KW - Gene expression
KW - Immunophenotype
KW - Mesenchymal stem cells
KW - PCR array
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U2 - 10.1016/j.vetimm.2014.06.002
DO - 10.1016/j.vetimm.2014.06.002
M3 - Article
C2 - 25026887
AN - SCOPUS:84906791661
VL - 161
SP - 21
EP - 31
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 1-2
ER -