Immunoperoxidase labeling of canine distemper virus replication cycle in Vero cells.

Robert Higgins, S. Krakowka, A. E. Metzler, A. Koestner

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An indirect immunocytochemical labeling technique, using horseradish peroxidase-conjugated antibody was used to detect the intracellular and surface membrane localization of canine distemper virus (R252-CDV) antigens during productive virus replication in infected Vero cells. Specific labeling of intracellular viral antigens was restricted to rough nucleocapsid aggregates. Surface membrane labeling correlated directly with the appearances both of virus-specific membrane spikes and buds and of mature virions. Syncytial cell formation was associated with labeled cytoplasmic nucleocapsid, but there was no evidence of productive CDV formation on surface membranes. The immunoperoxidase technique provided precise ultrastructural antigenic localization with concomitant preservation of excellent ultrastructural detail within single virus-infected cells during CDV replication cycle in vitro.

Original languageEnglish (US)
Pages (from-to)1820-1824
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume43
Issue number10
StatePublished - Oct 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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