Immunopathologic changes in the thymus during the acute stage of experimentally induced feline immunodeficiency virus infection in juvenile cats

Jennifer C. Woo, Gregg A. Dean, Niels C Pedersen, Peter F Moore

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Abstract

The feline thymus is a target organ and site of viral replication during the acute stage of feline immunodeficiency virus (FIV) infection. This was demonstrated by histologic, immunohistologic, flow cytometric, and virologic tests. Thymic lesions developed after 28 days postinoculation (p.i.) and included thymitis, premature cortical involution, and medullary B-cell hyperplasia with germinal center formation and epithelial distortion. Alterations in thymocyte subsets also developed. Fewer CD4+ CD8- cells were detected at 28 days p.i., while an increase in CD4- CD8+ cells resulted in an inversion of the thymic CD4/CD8 ratio of single-positive cells, similar to events in peripheral blood. Provirus was present in all thymocyte subpopulations including cortical CD1(hi), CD1(lo), and B cells. The CD1(hi) thymocyte proviral burden increased markedly after 56 days p.i., coincident with the presence of infiltrating inflammatory cells. Increased levels of provirus in the CD1(lo) thymocyte subpopulation were detected prior to 56 days p.i. This was likely due to inclusion of infected infiltrating inflammatory cells which could not be differentiated from mature, medullary thymocytes. Proviral levels in B cells also increased from 70 days p.i. Morphologic alterations, productive viral infection, and altered thymocyte subpopulations suggest that thymic function is compromised, thus contributing to the inability of FIV-infected cats to replenish the peripheral T-cell pool.

Original languageEnglish (US)
Pages (from-to)8632-8641
Number of pages10
JournalJournal of Virology
Volume71
Issue number11
StatePublished - Nov 1997

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Feline Immunodeficiency Virus
Feline immunodeficiency virus
thymocytes
Virus Diseases
Thymocytes
Thymus Gland
Cats
cats
infection
B-lymphocytes
proviruses
Proviruses
B-Lymphocytes
cells
CD4-CD8 Ratio
Germinal Center
Felidae
virus replication
hyperplasia
lesions (animal)

ASJC Scopus subject areas

  • Immunology

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Immunopathologic changes in the thymus during the acute stage of experimentally induced feline immunodeficiency virus infection in juvenile cats. / Woo, Jennifer C.; Dean, Gregg A.; Pedersen, Niels C; Moore, Peter F.

In: Journal of Virology, Vol. 71, No. 11, 11.1997, p. 8632-8641.

Research output: Contribution to journalArticle

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