Immunonutrition with Long-Chain Fatty Acids Prevents Activation of Macrophages in the Gut Wall

Friederike Eisner, Petra Jacob, Julia Stefanie Frick, Maximilian Feilitzsch, Julia Geisel, Mario H. Mueller, Markus A. Küper, Helen E Raybould, Ingmar Königsrainer, Jörg Glatzle

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: Immune cells and inflammatory mediators are released from the gastrointestinal tract into the mesenteric lymph during sepsis causing distant organ dysfunction. Recently, it was demonstrated that macrophages in the gut wall are controlled by the vagus nerve, the so-called cholinergic anti-inflammatory pathway. Aim: This study aims to investigate whether an enteral diet with lipid prevents the activation of leukocytes in the gut wall. Methods: Mesenteric lymph was obtained from rats, receiving an enteral infusion of glucose or glucose + lipid before and after lipopolysaccharide (LPS) injection. Immune cells in mesenteric lymph were analyzed with fluorescence-activated cell sorting before and after LPS injection. Mesenteric lymph leukocytes from rats receiving enteral glucose with or without lipid were stimulated in vitro with LPS and tumor necrosis factor (TNF)α was measured in the supernatant. Results: The release of macrophages from the gut during sepsis was not significantly different in animals enterally treated with glucose or lipid. However, the release of TNFα from mesenteric lymph leukocytes after in vitro LPS stimulation was more than 3-fold higher in the glucose group compared to the lipid-treated group. Conclusions: During sepsis, activated macrophages are released from the gut into mesenteric lymph. However, an enteral diet with lipid is able to suppress the inflammatory cytokine release from mesenteric lymph leukocytes.

Original languageEnglish (US)
Pages (from-to)853-859
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume15
Issue number5
DOIs
StatePublished - May 2011

Keywords

  • Cytokines
  • Immunonutrition
  • Mesenteric lymph
  • Sepsis
  • Vagus

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Fingerprint Dive into the research topics of 'Immunonutrition with Long-Chain Fatty Acids Prevents Activation of Macrophages in the Gut Wall'. Together they form a unique fingerprint.

  • Cite this

    Eisner, F., Jacob, P., Frick, J. S., Feilitzsch, M., Geisel, J., Mueller, M. H., Küper, M. A., Raybould, H. E., Königsrainer, I., & Glatzle, J. (2011). Immunonutrition with Long-Chain Fatty Acids Prevents Activation of Macrophages in the Gut Wall. Journal of Gastrointestinal Surgery, 15(5), 853-859. https://doi.org/10.1007/s11605-011-1431-z